氧化苦参碱软膏对小鼠慢性湿疹的影响

Effect of Oxymatrine Ointment on Mice with Chronic Eczema

目的:观察自制氧化苦参碱软膏对小鼠慢性湿疹的治疗作用,并初步探讨其作用机制。方法:32只慢性湿疹模型小鼠随机分为氧化苦参碱软膏组、空白模型组、空白软膏组、复方醋酸地塞米松乳膏组4组,用药组外用连续给药14d,第15天处死,剪取背部造模皮肤,制作病理组织学切片,观察其炎症改变情况,及读取炎症细胞计数。同时心脏取血,采用酶联免疫法(ELISA)检测血清中白细胞介素-4(IL-4)和白细胞介素-1β(IL-1β)水平。结果:氧化苦参碱软膏组和复方醋酸地塞米松乳膏组的炎症细胞计数均明显低于空白软膏组和空白模型组(P<0.05)。病理学结果显示,氧化苦参碱软膏可有效改善慢性湿疹小鼠模型出现的炎症症状,减少炎性细胞浸润均,减轻水肿症状,与复方醋酸地塞米松乳膏作用相似。分子免疫学方面,尚未观察到氧化苦参碱软膏对IL-4和IL-1β水平有明显影响(P>0.05)。结论:氧化苦参碱软膏具有一定的抗炎作用,对湿疹有一定的治疗作用,其作用机理有待进一步研究。

Objective： To observe the effect of oxymatrine ointment on chronic eczema in mice, and preliminarily explore the mechanism. Methods： Thirty-two Kunming mice were randomly divided into four groups including oxymatrine ointment group, blank model group, blank ointment group and positive drug group treated with compound dexamethasone acetate cream. Eczema skin was con- tinuously treated with drugs for 14 days. The mice were sacrificed on the 15th day, and the eczema skin was clipped from back to make histological sections. The changes of inflammation were observed, and the inflammatory cell count was obtained. Meanwhile, heart blood was collected, and serum was obtained by centrifugation. The serum levels of IL-4 and IL-113 were determined by ELISA. Re- suits： The inflammatory cell count in oxymatrine ointment group and the positive drug group was lower than that in the blank model group and the blank ointment group （P 〈 0.05）. The pathology results showed that oxymatrine ointment could improve chronic eczema symptoms, reduce the inflammatory cell infiltration and decrease edema, which was similar to the effects of compound dexamethasone acetate cream. In the respect of molecular immunology, the IL-4 and IL-1β levels in serum showed no significant changes in oxymatrine ointment group （P 〉 0.05 ）. Conclusion： Oxymatrine ointment has certain anti-inflammatory effect on eczema, and the mechanism needs to be studied further.