摘要
为了优化超声波协同酶法提取芦蒿多糖的工艺条件,采用中心组合设计方法(Box-Behnken design),建立以超声时间、超声温度、加酶量、酶解时间和液料比对芦蒿多糖得率的影响的二次回归模型。获得超声波协同酶法提取芦蒿多糖的最佳工艺条件为超声时间40 min、超声温度50°C、加酶量1.5%、酶解时间1 h、液料比40:1(m L·g^(-1)),在此条件下,芦蒿多糖的得率为37.19%。结果表明该回归模型有极显著性,可以作为芦蒿多糖提取工艺的回归分析和参数优化。
In order to optimize the extraction technology of polysaccharide from Artemisia selengensis Turcz. by ultrasonic assisted enzymatic method,the Box-Behnken design was used to establish the effects of ultrasonic time,ultrasonic temperature,enzyme dosage,enzyme solution time and liquid to solid ratio on Artemisia polysaccharide yield of quadratic regression model. The optimum extraction conditions of ultrasonic assisted enzymatic method extraction of polysaccharide from Artemisia selengensis Turcz. were ultrasonic time 40 min,ultrasonic temperature 50°C,enzyme dosage 1.5%,enzymolysis time 1 h,liquid-to-solid ratio of 40:1(m L·g-1),under these conditions,the extraction of Artemisia polysaccharide was 37.19%. The results showed that the regression model was significant,which could be used as the regression analysis and parameter optimization of the extraction technology of Artemisia polysaccharide.
出处
《植物生理学报》
CAS
CSCD
北大核心
2017年第1期126-132,共7页
Plant Physiology Journal
基金
国家高技术研究发展计划(2012AA021701)
国家自然科学基金(21376112)
江苏省自然科学基金(BK20141081)
山东省黄河三角洲生态环境重点实验室开放基金(2015-KFJJ04)
南京市环境科学与工程重点学科建设项目~~
关键词
芦蒿
多糖
超声波法
纤维素酶
中心组合设计方法
Artemisia selengensis Turcz
polysaccharide
ultrasonic method
cellulose
Box-Behnken design