河南省乙型肝炎病毒分型特征及小S蛋白主要亲水区变异特点分析

Analysis on Genotype Distribution and Mutation of Major Hydroponic Region of Hepatitis B Virus in Henan Province

为了解河南省乙型肝炎(乙肝)HBV基因型分布及其主蛋白抗原主要亲水区(MHR)氨基酸(aa)位点变异情况。本研究采集河南省2012年HBV流行病学调查的部分HBsAg和HBeAg阳性的血清样本,提取HBV DNA并进行序列扩增,测序得到s基因序列,利用Mega6.0软件比较分析。共得到HBVS基因序列50条,基因型分布B型为16.0%(8/50)、C型为84.0%(42/50)。血清型分布中,adrq+为HBV主要流行血清型,流行率为84.0%。S基因MHR aa位点变异中,T126变异率最高,为14.0%。HBV MHR变异株总流行率为24.0%(12/50),其中B型的突变率为37.5%(3/8),C型的突变率为21.4%(9/42)。河南省乙肝基因型分布以C型为主,B型次之。血清型主要为adrq+为主,adw2次之。HBV MHR aa位点存在变异,应在今后的计划免疫和HBIG治疗中给予重视。

To understand the genotype and the mutation of amino acid （aa） in the major hydrophilic region （MHR） of hepatitis B virus（HBV）. The serum samples were collected from the surveillance of HBV a- mong population in Henan in 2012. The S gene of HBV was amplified and sequenced. The acid amino se- quences were analyzed with Mega6.0 software. A total of 50 sequences of HBV S gene were contained, in- cluding 8 sequences of genotype B（16.0%） and 42 sequences of genotype C （84.0%）. The main serotype of HBV among Henan population was adrq＋ （84.0%） . The mutation rate of T1261 was the highest （14.0%）. The overall prevalence of mutant strain of MHR was 24.0% （12/50） ,and it was 37.5% （3/8） for genotype B,21.4% （9/42） for genotype C. HBV genotype C was predominant in Henan, followed by genotype B. The adrq＋ serotype was predominant followed by adw2. The mutation of amino acid in MHR of HBV was detected in this study. It is necessary to strengthen the surveillance for HBV mutation to pro- vide accurate information during immunization process and HBIG therapy.