冠心病痰瘀互结证小鼠模型的初步实验研究

Preliminary experimental study on the establishment and evaluation of mice model about coronary heart disease with phlegm and blood stasis syndrome

[目的]探讨冠心病痰瘀互结证小鼠模型的建立和评价方法。[方法]小鼠分为空白组:10只C57BL/6J小鼠,喂养普通饲料6周。痰瘀互结证模型组:10只ApoE基因敲除小鼠,喂养高脂饲料6周,并于小鼠处死前7 d每日皮下注射异丙肾上腺素(10 mg/kg),空白组给予等量的生理盐水。实验中观察小鼠的一般状态,第6周末检测小动物超声和血清中血脂水平,共同评价模型的建立。[结果]与空白组比较,痰瘀互结模型组小鼠舒张末期室间隔厚度(IVSd)、收缩末期室间隔厚度(IVSs)、舒张末期左心室后壁厚度(LVPWd)、收缩末期左室后壁厚度(LVPWs)均明显增厚(P<0.01),呈心腔扩大的心肌肥厚特征,左室质量(LVM)明显增大(P<0.05或P<0.01)。小鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)明显升高(P<0.05或P<0.01)。[结论]ApoE基因敲除小鼠通过高脂饲料喂养结合异丙肾上腺素诱导可以建立冠心病痰瘀互结证小鼠模型,模型稳定,易操作,重复性好。

[Objective] To establish and evaluation the method of mouse model about coronary heart disease with phlegm and blood stasis syndrome. [Methods] Mice were divided into blank group： 10 C57BL/6J mice, fed ordinary feed 6 weeks; phlegm and blood stasis syndrome model group：10 ApoE knockout mice, fed high fat diet for 6 weeks, and isoprenaline by subcutaneous injection （10 mg/kg） for 7 d before the mice to death, 1 time a day, and blank group was given the same amount of saline solution. To observe the general condition of mice in the experiments, the ultrasonic detecting of small animals and serum lipid levels were detected at the 6th weekend, than the model were evaluated. [Results] Compared with the blank group, phlegm and blood stasis syndrome model group mice IVSd, IVSs, LVPWd, LVPWs were thickening, and there were significant （P〈0.01）, and heart presented characteristics of myocardial hypertrophy, and LVM significantly increased（P〈0.05 or P〈0.01）. The serum TC, TG and LDL-C of mice increased significantly（P〈0.05 or P〈0.01）. [Conclusion] ApoE knockout mice fed by high fat feed combined with isoprenaline induced can establish coronary heart disease with phlegm and blood stasis syndrome of mouse model. The model is stable, easy to operate, and has good repeatability.