结直肠癌组织中KIAA1522的表达及临床意义

Significance in expression of KIAA1522 in colorectal cancer

目的:检测KIAA1522蛋白在结直肠癌中的表达变化并评价其临床意义。方法:应用组织芯片-免疫组织化学染色技术,检测96例结直肠癌组织及其配对癌旁正常组织中KIAA1522蛋白的表达情况,并对其阳性表达率与结直肠癌临床病理指标及患者预后的关系进行统计学分析。结果:KIAA1522蛋白在癌旁正常结直肠上皮细胞中主要定位于细胞浆,阳性表达率为12.5%(12/96),而在结直肠癌组织中KIAA1522蛋白阳性表达率为81.3%(78/96),两者间差异显著(P<0.05)。Kaplan-Meier生存分析结果显示,KIAA1522蛋白阳性表达与结直肠癌患者术后3年无瘤生存期短显著正相关(P=0.017,Log-rank检验)。多因素Cox回归分析结果表明,KIAA1522蛋白阳性表达是结直肠癌患者预后不良的独立预测因素(P=0.020)。卡方检验的分析结果显示,KIAA1522蛋白阳性表达与肿瘤的远处转移正相关(P=0.012,Fisher精确检验)。结论:KIAA1522在结直肠癌组织中的阳性表达与肿瘤的远处转移及患者术后生存期短显著相关,可能作为预测结直肠癌患者预后及转移的潜在分子标志。

OBJECTIVE：To investigateexpression status of KIAA1522 in colorectal cancer （CRC）,and its correlation with clinicopathological features and prognosis. METHODS：Expression of KIAA1522 protein was determined with tissue microarrays-immunohistochemistry （TMA-IHC） technique in surgically-resected colorectal cancer tissues and adjacent normal epithelia from 96 patients. The relationship between KIAA1522expressionand clinicopathological characteristics and prognosis of the patients were analyzed.RESULTS：KIAA1522 protein was mainly localized in the cytoplasm of colorectal epithelial cells. While only 12.5% （12/96） of normal colorectal epithelia displayed positive staining,81.3% （78/96） of CRC specimens showed over-expression of KIAA1522. The difference between them was highlysignificant （P〈0.001）. Kaplan-Meier survival analysis revealed that high level of KIAA1522 expression was correlated with lower 3-year disease free survival （DFS） rate of CRC patients （P=0.017）. Furthermore,KIAA1522 up-regulation was significantly correlated with distant metastasis （P=0.012）. More importantly,Cox regression analysis indicated that KIAA1522 protein expression was an independent prognosis factor in CRC （P=0.020）.CONCLUSION：KIAA1522 was frequently up-regulated in CRC,and its over-expression was significantly correlated with distant metastasis and poor prognostic,thus KIAA1522 over-expression may served as a new prognostic marker in colorectal cancer.