吉西他滨诱导自噬对HepG2细胞增殖和周期的影响

Effects of Autophagia Induced by Gemcitabine on HepG2 Cells Proliferation and Cycle

目的:研究吉西他滨诱导肝癌HepG2细胞自噬以及使用自噬抑制剂后对HepG2细胞增殖、周期的影响。方法:通过CCK-8法检测不同浓度的吉西他滨以及在吉西他滨的基础上加入自噬抑制剂后对HepG2细胞的增殖的影响。应用流式细胞术检测细胞周期各时相变化。结果:自噬诱导剂吉西他滨对HepG2细胞的抑制率明显高于对照组(P<0.05),同种情况下加入一定量的自噬抑制剂3-甲基嘌呤(3-methyladenine,3-MA)后,培养24h后,细胞的抑制率与吉西他滨诱导组相比较明显升高(P<0.05)。流式细胞术的结果显示吉西他滨组与对照组相比较细胞周期中G1期细胞明显增多,S期明显减少;加入自噬诱导剂后G1期细胞明显减少,S期细胞明显增多(P<0.05)。结论:吉西他滨诱导肝癌HepG2细胞发生自噬对细胞的增殖有明显的抑制作用,并且吉西他滨诱导肝癌HepG2细胞发生自噬多停留在细胞周期的G1期。

Objective：To study the effects of autophagia induced by gemcitabine of HepG2 cells on cell proliferation and cell cycle. Methods：CCK-8 assay was used to detect the effects of different concentrations of gemcitabine on the proliferation of HepG2 cells and another condition with the addition of autophagy inhibitors. Flow cytometry was used to detect the changes of ceil cycle. Results ：The in- hibition of gemcitabine group on HepG2 cells was significantly higher than that of control group（P〈0. 05）, under the same conditions adding a certain amount of autophagy inhibitor 3-（3-methyladenine, 3-MA） methyl purine, after cultured 24 h, the inhibition rate increased significantly compared to the gemcitabine group（P〈0. 05）. The result of flow cytometry showed that compared with the control group, gemcitabine group G1 phase cells increased significantly while S phase cells decreased significantly; G1 phase cells decreased significantly with autophagy inducer while S phase cells increased significantly （P 〈 0. 05 ）. Conclusion： The autophagy of HepG2 ceils induced by gemcitabine can inhibit the proliferation of the cells,and the autophagy of HepG2 cells induced by gemcitabine can mostly be observed in the G1 phase of the cell cycle.