功能蛋白质组学在神经系统疾病中的应用

Application of functional proteomics in neurological disorders

随着生命科学的快速发展,定量蛋白质质谱技术应运而生,在基础科学研究中发挥越来越重要的作用。质谱技术检测蛋白质差异表达、蛋白质翻译后修饰以及蛋白质之间的相互作用,在不同的生物系统中解决不同的生物学问题,在临床诊断中也发挥着重要的作用。随着人类社会的进步,社会及自然环境的改变,神经系统疾病的发病率逐年增加。大脑作为人类认知所特有的器官,在神经系统疾病中作为主要研究对象,具有结构的复杂性及组织功能性的严密性,对其研究更需要高产出,更系统的研究方法。质谱技术的高精确性及高灵敏度很好地满足这一需求,因此本文主要介绍基于"鸟枪法"的串联质谱技术在神经系统疾病的发病机理及临床诊断中的重要应用,并对目前的研究现状做一个展望。

With the rapid advancement of life science, mass spectrometry-based proteomics has played an increasingly pivotal role in basic and clinical research. This technique allows studying differential protein ex- pression, posttranslational modifications and protein-protein interactions. Human society has moved forward with an ever-changing social and natural environment, neurological disorders have occurred with an increas- ing frequency. The ultimate thinking organ, the brain, is a major object of study in neurological disorders. Our brain is the mixture of complex histological structures and functional organization. To solve the complexity of the brain, mass spectrometry technique can meet this demand due to its high resolution and sensitivity. This review will focus on introducing application of shotgun-proteomics-based tandem mass spectrometry in the research on pathogenic mechanism and clinical diagnosis of neurological disorders.