摘要
用RAPD技术对蚱属 5种蚱基因组DNA的多态性进行研究。在事先优化的反应条件下用 12个随机引物扩增 ,共得到84条清晰稳定的多态性片段 ,片段长度为 2 0 0～ 2 0 0 0bp。统计这些片段 ,根据扩增片段的共享度计算出相对遗传距离指数 ,然后用UPGMA和NJ聚类方法对其进行分析 ,构建系统树 。
Random amplified polymorphic DNA(RAPD)techniques were applied to assess genetic variations among five species in the genus Tetrix. Amplifications with 12 primers under predetermined optimal reaction conditions gave 84 reproducible fragments ranging from 200 to 2 000 bp.The amplified products were scored as present (1) or absent (0) for each DNA sample and an index of genetic similarity (F) was calculated. The value of D was used to evaluate genetic distances between species and a phylogenetic tree was constructed.
出处
《昆虫学报》
CAS
CSCD
北大核心
2002年第4期499-502,共4页
Acta Entomologica Sinica
基金
广西青年科学基金资助项目 (桂科青 0 0 0 70 14 )
