盐酸米诺环素原位凝胶的制备及其对牙周炎大鼠的治疗效果

Preparation of minocycline hydrochloride-loaded in-situ hydrogels and effect on the periodontitis of rats

目的评价盐酸米诺环素原位凝胶对牙周炎大鼠的治疗效果。方法结扎/高糖饮食/局部接种牙龈卟啉单胞菌/肌肉注射醋酸泼尼松龙联合因素建立牙周炎大鼠模型,4周后根据临床检查和病理切片确定牙周炎模型是否建立成功。将28只牙周炎模型大鼠分为4组:模型组、阳性对照药(盐酸米诺环素软膏,派丽奥)组、自制空白凝胶组和自制盐酸米诺环素原位凝胶组,分别在治疗前和治疗后4周检查牙龈指数、出血指数、探诊出血和探诊深度4项临床指标。结果牙周炎大鼠经盐酸米诺环素软膏和盐酸米诺环素原位凝胶治疗后,牙周组织炎症程度及牙周破坏程度比模型组和空白凝胶组减轻。结论盐酸米诺环素原位凝胶治疗大鼠牙周炎疗效明显,有望成为一种新型牙周炎治疗制剂。

Objective To evaluate the effect of minoeycline hydrochloride （MH）-loaded in-situ hydrogels on periodontitis of rats. Methods Ligation, high sugar diet, local vaccination of Porphyromonas gingivalis and intramusclar injection of acetic prednisolone were combined to establish the periodontitis of rats. Whether periodontitis was established successfully or not was determined according to clinical examination and pathology after 4 weeks. 28 periodontitis rats were divided into the model group, the positive control （MH ointment ）, the blank in-situ hydrogels （ISG） and MH-loaded ISG groups. The detection index included gingival index, bleeding index, bleeding on probing, and probing depth before treatment and 4 weeks after treatment, respectively. Results The inflammatory and destruction degrees of periodontal tissues in the positive and MH ISG groups were better than those of the model and the blank groups. Conclusion The curative effect of MH-loaded ISG on periodontitis is obvious. It is expected to become a novel formulation to treat periodontitis in future.