HPLC法同时测定升麻葛根汤中3个有效成分的含量

Simultaneous determination of three active components in decoction of rhizoma cimicifugae and radix puerariae by HPLC

[目的]用高效液相色谱(HPLC)法,建立一种能同时测定升麻葛根汤汤剂中异阿魏酸、葛根素和芍药苷含量的分析方法。[方法]Waters XBridge~Shield RP18色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.05 mol/L磷酸二氢钾为流动相,梯度洗脱,体积流量为0.8 mL/min,双波长检测,检测波长分别为230 nm、317nm,柱温30℃。[结果]芍药苷、葛根素和异阿魏酸在该色谱条件下能分离良好,且阴性无干扰。其中芍药苷、葛根素和异阿魏酸的线性范围分别为0.382~6.118μg、1.602~25.626μg和0.081~1.303μg;平均回收率(n=6)分别为101.5%、102.9%和98.1%,RSD均小于3.0%。[结论]本方法操作简便、准确、重复性好,可同时测定升麻葛根汤中芍药苷、葛根素和异阿魏酸的含量,为升麻葛根汤的质量评价提供科学依据。

[Objective] To develop an analytical method for simultaneously determining the contents of paeoniflorin, puerarin andisoferulic acid in decoction of rhizoma cimicifugae and radix puerariae by HPLC. [Methods] The separation of three active components were performed on a Waters XBridge column （250 mm×4.6 mm, 5 μ m） by using an gradient mobile phase composed of CH3OH -0 .0 5mol/L KH2PO4 , at acolumn temperature of 30 ℃, the flow rate was 0.8 mL/min, the detection wavelength was 230 nm and 317 nm. [Results ] The peaks of paeoniflorin, puerarin and isoferulic acidcompletely resolved very well. The linear ranges of paeoniflorin, puerarin and isoferulic acid were 0.382 - 6.118 μ g, 1.602 - 25.626 μg and 0.081 - 1.303 μg, the average recoveries （n =6 ） were 101.5%, 102.9% and 98.1% respectively, and the RSD was less than 3.0%. [Conclusion] This method is simple, accurate and repeatable. It can be used to simultaneously determine the content of the above three active compounds, as well as to provide a scientific basis for the quality evaluation of the decoction.