前列腺癌患者血清和病理组织中miRNA-375的表达及意义

Expression and clinical significance of serum miRNA-375 in patients with prostate cancer

目的探讨前列腺癌患者血清和病理组织中miRNA-375的表达及临床意义。方法选取2015年3月至2016年3月间我院收治的前列腺癌患者30例为观察组,另选择同期前列腺增生患者30例为对照组,检测并比较两组患者血清及病理组织中miRNA-375变化,观察血清miRNA-375表达与临床病理关系,分析病理组织与血清中mi-RNA-375相关性及前列腺癌患者血清miRNA-375与血清PSA的相关性。结果与前列腺增生患者比较,前列腺癌患者PCR定量检测miRNA-375血清中2-△Ct显著增高,病理组织中miRNA-375表达显著升高,差异有统计学意义(P<0.05)。病理分期T3~T4期、Gleason评分>7分、存在转移及PSA>10μg/L者显著高于病理分期T1~T2期、Gleason评分≤7分、无转移及PSA≤10μg/L者,差异有统计学意义(P<0.05)。前列腺癌患者mi-RNA-375与血清PSA呈线性相关(r=0.714,P<0.05)。前列腺癌病理组织与血清中mi-RNA-375呈线性相关(r=0.911,P<0.05)。结论前列腺癌患者血清miRNA-375水平存在明显升高,且与患者临床病理特征存在相关性。

Objective To investigate the changes and clinical significance of serum miRNA-375 in patients with prostate cancer. Methods From March 2015 to March 2016,30 patients with prostate cancer were selected as the observation group,and 30 patients with benign prostatic hyperplasia（ BPH） were selected as the control group. The changes of miRNA-375 in serum and pathological tissues were detected and compared between the two groups. The relationship between the expression of serum miRNA-375 and clinical pathology,the correlation between pathological tissues and serum,and the correlation between serum miRNA-375 and serum PSA were observed and analyzed. Results Compared with BPH patients,serum miRNA-375 2-△Ct detected by quantitative PCR and the expression of miRNA-375 in pathological tissues significantly increased in patients with prostate cancer（ P〈0. 05）. The expression of miRNA-375 were significantly higher in pathological T3-T4 stage,Gleason score〉 7,metastasis and PSA〉 10 μg/L patients than in pathological T1-T2 stage,Gleason score ≤7,non-metastasis and PSA≤10μg/l patients（ P〈0. 05）. MiRNA-375 in prostate cancer patients was linearly correlated with serum PSA（ r = 0. 714,P〈0. 05）. Prostate tissue was linearly correlated with serum mi-RNA-375 in in patients with prostate cancer（ r = 0. 911,P〈0. 05）. Conclusion The level of serum miRNA-375 in patients with prostate cancer was significantly increased,which was correlated with clinical pathological characteristics.