天津地区发生禽心包积液-肝炎综合征及其病毒分离鉴定

Hydropericardium Syndrome-Inclusion Body Hepatitis in Tianjin Area and Virus Isolation & Identification

为探究禽心包积液-肝炎综合征的发病原因。对2016年7月天津市某家禽养殖场的病死鸡进行了大体病变检查、组织病理学检查、细菌学检验及其病毒的分离鉴定和回归试验等。结果显示,剖检以大量心包积液和肝脏肿大为主;组织切片H.E.染色观察到肝脏淤血、水肿,肝细胞变性、坏死且含有大量嗜碱性核内包涵体;细菌培养均为阴性;PCR检测禽腺病毒-4型(FAd V-4)呈现阳性;回归试验的结果与临床病例一致。最终诊断为禽心包积液-肝炎综合征,与I群4型禽腺病毒相关。病毒的致病性研究正在进行中。

In order to explore the causes of hydropericardium syndrome-inclusion body hepatitis,experiments using the methods of symptom examination,gross lesion histopathological observation,bacteriologic tests,virus isolation and artificial infection were applied on a chicken farms in Tianjin in July 2016. The results showed that pericardial enlarged hydropericardium and hepatomegaly were the main changes grossly. Congestion and edema in liver,hepatocytic degeneration and necrosis with many intranucleic basophilic inclusion bodies in tissue sections stained with H. E. were observed under microscope. Bacterial cultures were negative and FAd V-4 was positive by PCR. Artificial infection case was the same as clinical case. These results suggest that hydropericardium syndrome-inclusion body hepatitis is caused by FAd V-4 infection. The virus pathogenicity studies are ongoing.