LncRNA GAS5对结肠癌细胞生长及化疗药物作用的影响

Effect of LncRNA GAS5 on cancer cell survival and chemosensitivity in colon cancer

目的检测长链非编码RNA生长抑制特异因子5(LncRNA GAS5)对结肠癌细胞的凋亡和生长的影响及其与化疗药物的敏感性是否有关联。方法 siRNA干扰结肠癌细胞株SW480 LncRNA GAS5基因,检测细胞的存活率,加入化疗药物5氟尿嘧啶(5-FU),检测细胞的克隆形成能力,流式细胞仪检测干扰前后细胞的凋亡。结果 siRNA干扰结肠癌细胞SW480 LncRNA GAS5基因后,加入化疗药物5氟尿嘧啶,敲除GAS5基因后癌细胞存活率比敲除GAS5基因前多,其差异具有统计学意义(P<0.05);敲除GAS5基因后,结肠癌细胞的克隆形成能力比敲除GAS5基因前强,其差异具有统计学意义(P<0.05);流式细胞仪检测敲除GAS5前后的结肠癌细胞,敲除GAS5后结肠癌细胞凋亡数量降低,其差异具有统计学意义(P<0.05)。结论 GAS5可促进结肠癌细胞的凋亡;GAS5能促进化疗药物对结肠癌细胞的作用;GAS5可能作为抑癌基因存在于结肠癌细胞中,为结肠癌的分子靶向治疗提供依据。

Objective To investigate the effect of LncRNA GAS5 （growth arrest-specific 5） on apoptosis, cell viability and chemosensitivity of colon cancer. Methods siRNA was used to interfere the expression of LncRNAs GAS5 in SW480 cell line. And the cell survival rate of the cells and the difference of the colony formation ability of the cells after 5 fluorouracil treatment was analyzed. Flow cytometry were introduced to evaluate the function of LncRNA GAS5 on apoptosis and chemosensifivity to 5 fluorouracil. Results After siRNA inference with SW480 LncRNA GASS, 5 fluorouracil treatment demonstrated cell viability significantly increased after GAS5 knockdown in SW480 cells compared with that before GAS5 knockdown （P〈0.05）. Cell apoptosis rate reduced after GAS5 knockdown in SW480 cells （P〈0.05）. Meanwhile flow cytometry detection showed colony formation ability significantly promoted （P〈 0.05）. Conclusion GAS5 could promote colon cancer cell apoptosis. GAS5 could promote the chemosensitivity of co- lon cancer cells to 5 fluorouracil. GAS5 may act as a tumor suppressor gene exists in colon cancer cells, and provide a new molecular therapy target for colon cancer.