山羊BMPR-IB基因编码蛋白结构及功能的生物信息学分析

Bioinformatics Analysis of the Structure and Function of BMPR-IB Gene Coding Protein in Goat

试验旨在利用生物信息学方法分析山羊骨形态发生蛋白受体IB(bone morphogenetic protein receptor IB,BMPR-IB)基因编码蛋白的结构及功能。本研究通过在线软件预测和分析山羊BMPR-IB基因编码蛋白的一级结构、二级结构、亚细胞定位、三级结构、重要的功能基序及其分类,通过最大似然法对不同物种BMPR-IB基因编码蛋白序列构建系统发育树,进行系统发育分析。结果发现,山羊BMPR-IB基因共编码502个氨基酸,其编码蛋白属于不稳定的亲水性蛋白质。二级结构以β折叠为主,比例为63.5%,主要存在于细胞核中,在线粒体、细胞质、囊泡分泌系统和质膜中也有少量分布。该蛋白主要发挥嘌呤和嘧啶、监管、运输和结合、信号转导等功能。在127-149氨基酸位置存在1个跨膜结构域;在174-203、204-494氨基酸位置存在2个高度保守的基序,分别为GS结构域和蛋白激酶结构域。三级结构由sheet片段结构富集域和helix螺旋结构富集域组成。不同物种BMPR-IB基因系统发育分析结果与动物学分类结果一致,提示BMPR-IB基因与繁殖力性状存在联系。通过不同的在线预测软件分析了山羊BMPR-IB基因编码蛋白的结构及功能,为深入研究BMPR-IB基因提供理论指导。

This study was aimed to analyze the structure and function of BMPR IB gene coding protein by bioinformatics.The primary structure,secondary structure,subcellular localization,tertiary structure,important functional motifs and functional classification of BMPR-IB gene coding protein in goat were predicted and analyzed by online softwares.Phylogenetic tree of BMPR-IB gene coding proteins of different species were constructed by maximum likelihood method and phylogenetic analysis was performed.The results showed that the goat BMPR-IB gene encoded 502 amino acids,its coding protein belonged to an unstable hydrophilic protein.Secondary structure was mainly beta-sheet（63.5%）.The protein was mainly located in the nucleus,and also distributed in mitochondria,cytoplasm,vesicle secretion system and plasma membrane.The proteins mainly played the roles of purine and pyrimidine,regulation,transport and binding,signal transduction and so on.There were one transmembrane domains in the location of127 th to 149 th amino acids,the GS domain in the location of 174 th to 203 th amino acids and the protein kinase domain in the location of 204 th to 494 th amino acids were highly conserved motifs.The tertiary structure consisted of sheet fragment structure enrichment domain and helix helical structure enrichment domain.The results of phylogenetic analysis of BMPR-IB gene in different species was consistent with the results of animal taxonomy,and suggested that BMPR-IB genewas associated with the fertility traits.The structure and function of BMPR-IBgene coding protein in goat were analyzed by different online prediction software,which provided theoretical guidance for further study of BMPR-IB gene.