铁皮石斛多糖的提取工艺及其治疗原发性干燥综合征疗效观察

Optimal extraction procedure of polysaccharides from tin caulis dendrobii and therapeutic effects of polysaccharides on patients with primary Sjgren's syndrome

目的:对铁皮石斛多糖的提取工艺进行优化研究,并分析铁皮石斛多糖治疗原发性干燥综合征的疗效。方法:运用超生粉碎法提取铁皮石斛多糖,并选取不同浸提时间(A)、提取次数(B)、不同固液比(C)3个因素进行正交试验,以分析铁皮石斛多糖提取的最佳工艺参数。选取原发性干燥综合征患者30例,分为治疗组和对照组各15例,分别给予铁皮石斛多糖和硫酸羟氯喹治疗,并对比分析治疗前后疗效、唾液流量及血常规。结果:正交试验结果表明,提取的最佳工艺为提取时间0.5 h,提取8次,固液比1:20。与治疗前比较,治疗后两组患者静态唾液流量都有所升高,且铁皮石斛多糖治疗组患者静态唾液流量升高较多。治疗组(铁皮石斛多糖)总有效率是80%,对照组(硫酸羟氯喹组)总有效率是73.33%。结论:铁皮石斛多糖能够改善干燥综合征患者症状,且改善口干症状方面疗效显著。

Objective：To establish optimal extraction procedure of polysaccharides from tin caulis dendrobii and ob- serve its curative effects on patients with primary Sjgren＇ s syndrome. Methods： Orthogonal design was adopted, and time and times of boiling, solid-fluid ratio were selected as variable factors. The effects of patients with primary Sjgren＇ s syndrome were observed by measuring the total curative effect, saliva flow and routine blood. Results：The most favorable extraction condi- tions were as follows, boiling 8 times and 0. 5 hours each time with solid-fluid ratio of 1 ： 20. Compared with before treat- ment, the static flow was increased in the two groups after treatment, and the polysaccharides groups increased more than the hydroxychloroquine sulfate group. The treatment efficiency of the polysaccharides group was 80% , and the efficiency of the hydroxychloroquine sulfate group was 73.33%. Conclusion： Polysaccharides from tin caulis dendrobii could improve the symptoms of patients with primary Sjgren＇ s syndrome.