宫颈脱落细胞HPV L1蛋白及HPV DNA载量在宫颈病变中的临床意义

Clinical Significance of HPV L1 Protein and HPV DNA Load of Cervical Exfoliated Cells in Cervical Lesions

目的探讨宫颈脱落细胞HPV L1蛋白及HPV DNA载量在宫颈病变中的临床意义。方法收集我院2014年5月至2016年1月的237例患者的宫颈脱落细胞标本,检测其HPV DNA载量,并对高危型HPV阳性者行HPV L1蛋白检测,分析检测结果。结果 HPV L1蛋白阳性率为21.52%(51例),正常/慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌患者的HPV L1蛋白阳性率比较差异有统计学意义(P<0.05)。HPV阳性率为74.26%(176例),正常/慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌患者的HPV阳性率比较差异有统计学意义(P<0.05)。HPV DNA载量低度组、中度组、高度组的HPV L1蛋白阳性率分别为39.22%、51.95%、77.08%,高度组的HPV L1蛋白阳性率显著高于其他两组,差异有统计学意义(P<0.05)。结论 HPV L1蛋白与HPV DNA载量对评估宫颈病变有一定作用,对其进行监测能够为宫颈病变预测、治疗及预后提供有效参考。

Objective To explore the clinical significance of HPV L1 protein and HPV DNA load of cervical exfoliated cells in cervical lesions. Methods 237 samples of cervical exfoliated ceils of patients in our hospital from May 2014 to January 2016 were collected. The HPV DNA load was detected, and the L1 HPV protein of high-risk HPV positive patients were detected. The detection results were analyzed. Results The HPV L1 protein positive rate was 21.52% （51 cases）. Statistical difference was found in the HPV L1 protein positive rates of patients with normal/chronic cervicitis, C1N Ⅰ and C1N Ⅱ, C1N Ⅲ and cervical cancer （P〈0.05）. The HPV positive rate was 74.26% （176 cases）. Statistical difference was found in the HPV positive rates of patients with norma/chronic cervicitis, C1N Ⅰ and C1N Ⅱ, C1N Ⅲ and cervical cancer （P 〈0.05）. The HPV L1 protein positive rate of low HPV DNA load group, medium group and high group were respectively 39.22%, 51.95% and 77.08%. The HPV L1 protein positive rate of high group was significantly higher than that of other groups, with statistical differences （P〈0.05）. Conclusions HPV L1 protein and HPV DNA load has certain effect in the evaluation of cervical lesions. Monitoring both indicators can provide effective reference for prediction, treatment and prognosis of cervical lesions.