牛肉及其制品中掺入鸡肉、鸭肉和猪肉的多重数字PCR快速检测方法研究

Rapid detection of chicken,duck and pork blending in beef products by multiple digital PCR

利用微滴式数字PCR,建立针对牛、猪、鸡和鸭肉的多重数字PCR检测技术。结果表明,所选择的4个物种的引物和探针特异性良好,能够快速对样品中是否存在这4种肉类成分做出快速判断。数字PCR分析中,FAM和HEX两个通道的荧光信号能很好地区分,在对9个实际样品的检测中,所在样品的微滴生成数都在10 000以上,满足数字PCR中泊松公式分析的要求,同时9个样品的检测结果与已知信息完全相符。

In this study,a quickly detective method,using droplet digital PCR,for detection of beef,chicken,duck and pork was established. The results showed that the selected primers and probes for the four species had good specificity,and could quickly make out these four kinds of mean component in tested samples. In all of the digital PCR analysis,the fluorescence signals of FAM and HEX can be well distinguished. For detection of 9 samples,the numbers of droplets generated in the multiple digital PCR were all above 10 000,which could meet the requirements for the poisson formula analysis in digital PCR. meanwhile,the test results of the 9 samples using multiple digital were consistent with the known information of these samples.