大肠癌组织中CDK6、Ki67表达及其与临床病理因素的关系

Expression of CDK6 and Ki67 and their relation to clinicopathological features in colorectal cancer

目的:探讨大肠癌组织中CDK6、Ki67蛋白的表达及与其大肠癌病理因素的关系及二者之间的相关性。方法:应用免疫组织化学方法研究84例大肠癌组织及其配对的48例切缘正常大肠组织中CDK6、Ki67蛋白的表达情况,比较CDK6、Ki67表达的差异及其与大肠癌病理因素之间的关系及二者之间的相关性。结果:大肠癌组织中CDK6蛋白表达阳性率(88.1%)明显高于切缘正常大肠组织(10.4%)(P<0.05),CDK6蛋白表达阳性率与Dukes分期、浸润深度、淋巴结转移、肿瘤分化程度有关(P<0.05);与患者年龄、性别、肿瘤发生部位、肿瘤大体类型无关(P>0.05);大肠癌组织中Ki67蛋白表达阳性率(92.9%)明显高于切缘正常大肠组织(20.8%)(P<0.05),Ki67蛋白表达与患者年龄、性别、肿瘤发生部位、肿瘤大体类型、Dukes分期、浸润深度、淋巴结转移、肿瘤分化程度无关(P>0.05),CDK6与Ki67蛋白表达具有正相关关系(r=0.581,P=0.000)。结论:CDK6与Ki67过量表达可能在大肠癌的发生、发展过程中起重要作用,可能用于大肠癌辅助诊断。CDK6可作为判断大肠癌预后的因子之一。

Objective The present study was designed to investigate the expression of CDK6 & Ki67 protein,as well as their relationship to the clinicopathological features in colorectal cancer,and the corelation of CDK6 & Ki67.Methods Samples of the 132 cases were divided into 2 groups:84 cases of colorectal cancer tissues,48 cases of incisal edge normal colorectal tissues,Immunohistochernical method was applied to detect the expression of CDK6 & Ki67 protein in tissues.Analyzed the expression of CDK6 & Ki67 in the tissues in group of colorectal cancer and group of normal controls.And analyzed the differential expression of CDK6 & Ki67 in colorectal cancer and its relationship with colorectal cancer in clinical and pathological features.Analyzed the corelation of CDK6 & Ki67.Results The positive rate of CDK6 protein expression in colorectal cancer tissue and in normal controls is 88.1%,10.4% respectively.The positive rate of CDK6 protein expression shows significant different between colorectal cancer tissue and normal colorectal tissue(P < 0.05),The positive rate of CDK6 protein expression in colorectal cancer tissue have obvious correlation with clinical and pathological festures such as Dukes stage,invasive depth,lymphatic metastasis,pathological differentiation degree(P < 0.05);and have no obvious correlation with patients'age,sex,location,general type(P > 0.05);The positive rate of Ki67 expression in colorectal cancer tissue and in normal controls is 92.9%,20.8% respectively.The positive rate of Ki67 protein expression shows significant different between colorectal cancer tissue and normal colorectal tissue(P < 0.05),The positive rate of Ki67 protein expression in colorectal cancer tissue have no obvious correlation with clinical and pathological festures such as patients'Dukes stage,invasive depth,lymphatic metastasis,pathological differentiation degree,age,sex,location,general type(P > 0.05);The positive rate of CDK6 & Ki67 protein expression in colorectal cancer tissue have positive correlation(r=0.581,P=0.000).Conclusion The high expression of CDK6 and Ki67 protein may be playing an important role in the development process of colon cance,CDK6 and Ki67 can be applied to the auxiliary diagnosis of large intestine cancer.CDK6 can be used as one of the prognostic factor to judge the prognosis of patients with colorectal cancer.