杨树NAC7转录因子基因应答盐胁迫表达

Expression Analysis of NAC7 Transcription Factor Gene from Populus simonii × P. nigra in Response to Salt Stress

从小黑杨中克隆915 bp的NAC7转录因子基因(Potri.007G099400.1)cDNA,其编码304氨基酸,该蛋白属热稳定性较高的亲水性蛋白。用RT-qPCR分析杨树盐胁迫条件下NAC7基因表达情况,表明该基因对盐胁迫具有应答反应,且基因主要在根部表达。克隆获得1 062 bp的NAC7基因启动子DNA序列,其中含有许多胁迫应答元件,其驱动的GUS报告基因主要集中在根中表达,而在茎和叶中的表达很少,这个结果与NAC7基因表达进行RT-qPCR分析结果一致。

The 915 bp cDNA fragment of NAC7 transcription factor gene （Potri.007G099400.1） was cloned from Populus nigra, which encodes 304 amino acids. The expression analysis of NAC7 gene in poplar under salt stress by RT-qPCR showed that the gene had response to salt stress. The 1 062 bp DNA fragment of NAC7 gene promoter was eloned, which contained many stress responsive elements, the GUS gene driven by NAC7 gene promoter mainly expressed in root, while the expression was rarely in leaves and stems, and the result was consistent with RT-qPCR of NAC7 gene.