氦氖激光对兔退变颞下颌关节髁突软骨基质表达的影响

Effects of He-Ne laser on expression of extracellular matrix in degenerated temporomandibular joint of rabbit

目的:通过研究氦氖激光对退变颞下颌关节髁突软骨细胞外基质基质胶原2(Col-2)、蛋白多糖4(PRG-4)表达的影响,明确氦氖激光对颞下颌关节退变的保护作用与机制,为临床运用氦氖激光治疗颞下颌关节骨关节病提供依据。方法:将骨骼发育成熟的健康成年雄性新西兰大白兔随机分为正常组、假模型组、模型组和干预组,每组10只,组内又分为造模后第1天、第11天两个取材时间点。参照常嘉等的造模方法,建立颞下颌关节骨关节病动物模型并行CT检查证实造模成功后纳入。干预组采用氦氖激光照射患侧颊车、上关、下关、听宫、翳风穴,5min/穴/d,共10d;假模型组仅作关节囊切开后即缝合组织。各组于不同时间点取出颞下颌关节组织,采用HE染色观察干预前后形态学改变;Western Blot方法检测Col-2、PRG-4的表达水平。结果:退变髁突软骨HE染色可见纤维降解,增殖层及过度层细胞排列紊乱,细胞核出现皱缩,核浓染,经过氦氖激光治疗后的髁突软骨纤维层轻度松解,部分纤维合成,红染增强。Western Blot检测发现正常组及假模型组不同时间点之间Col-2、PRG-4蛋白表达差异无显著性意义;模型组第11天各细胞外基质蛋白表达明显降低;氦氖激光干预10天后各细胞外基质蛋白的表达明显增加。结论:氦氖激光干预可上调退变颞下颌关节软骨细胞外基质Col-2、PRG-4蛋白表达,纠正细胞外基质降解,从而防治颞下颌关节骨关节病。

Objective： To observe effects of He-Ne laser on the expression of Col-2 and PRG-4 in degenerated temporomandibular joint of rabbit, and to explore its protective role and mechanism in relieving temporomandibular joint degeneration and provide the basis for clinical application of He Ne laser in the treatment of temporomandibular joint osteoarthritis.Method： Forty New Zealand white rabbits were randomly divided into 4 groups： normal group, sham model group, model group and treatment group, 10 in each group, every group was divided into two time points（1d,11d）. Establish animal model of temporomandibular joint disease consulting the building methods of Changjia.He-Ne laser was rayed at Jiache, Shangguan, Xiaguan, Tinggong, Yifeng point for 10 days in the treatment group. Stitch joint capsule as soon as its incision in sham model group. The animals were killed at day 1 and day 11 and HE staining was used to observe the morphological changes after treatment, the protein expression of extracellular matrix in temporomandibular joints were examined with Western Blot.Result： Degenerative condylar cartilage HE staining showed dyed fibers degradation, cells in proliferation and excessive layer arranged disorder, the nucleus in shrinking and hyperchromatic. After He-Ne laser treatment,the fibers in fiber layer of condylar cartilage mild soluted, part of the fibers composite and stained red. Col-2,PRG-4 protein expression had no statistical significance between different time point in the normal group and the sham model group by Western Blot test, but protein in extracellular matrix were up-regulated in the treatment group after 10 days He-Ne laser treatment.Conclusion： He-Ne laser on acupoints may protect temporomandibular joint from degeneration of protein in extracellular matrix through up-regulating the expression of Col-2, PRG-4.