一株猪源多杀性巴氏杆菌的分离鉴定及药敏试验

Isolation and identification of one Pasteurella multocida strain from swine and its drug sensitive test

采用分离培养、生化鉴定、16S r RNA扩增片段同源性分析等方法,对采自四川某规模化猪场有明显呼吸道症状的猪肺脏拭子进行细菌的分离鉴定,并进行药敏试验及毒力试验。结果显示,分离得到1株多杀性巴氏杆菌,该分离株对头孢呋辛、头孢曲松、阿米卡星等19种抗生素敏感,对氨苄西林和麦迪霉素中度敏感,对苯唑西林表现耐药。小鼠毒性试验显示,该菌株对小鼠有强致病性。本研究为猪巴氏杆菌病的防控提供参考。

One bacterial strain was isolated from the lung swab of pig with an obvious respiratory symptom in a large-scale pig farm of Sichuan. The bacterium was identified based on the morphological observation,biochemical assay,and 16 S rRNA sequence analysis. The sensitive drugs and virulence were also determined. The result showed that the bacterial strain was demonstrated to be Pasteurella multocida. It was sensitive to 19 antibiotics including cefuroxime,ceftriaxone and amikacin,moderately sensitive to ampicillin and medemycin,but resistant to oxacillin. And the bacterial strain was highly virulent. The study provides reference data for the prevention and control of P. multocida infection.