庞氏安胎汤对先兆流产大鼠的影响

Effect of Pongshi Antai Decoction on Threatened Abortion Rats

目的:通过灌服中药庞氏安胎汤和西药地屈孕酮,观察先兆流产大鼠的一般情况包括精神状态、皮毛的光泽度、活动力、阴道出血情况,有无妊娠物的排出等。观察各组孕鼠流产率的多少,研究庞氏安胎汤对先兆流产大鼠的影响。方法:将SD雌鼠与雄鼠配对,筛选出孕鼠(孕1天),随机分为正常组、模型先兆流产组、西药组、中药低剂量组、中药中剂量组、中药高剂量组6组,每组10只。通过灌胃西药、中药12 d后,孕12天上午8∶00除正常组外其他组灌服米非司酮,建立先兆流产大鼠模型,然后开始观察各组孕鼠一般情况,观察精神状态、皮毛光泽、活动度、阴道出血情况,有无妊娠物排出情况。孕13天处死各组大鼠,解剖各组孕鼠,观察各组胎鼠形态、大小,仔细记录着床数、活胎数、死胎数,计算流产率,观察庞氏安胎汤对先兆流产的影响。结果:(1)成功复制先兆流产模型:模型组流产率明显高于正常组(P<0.01);模型组大鼠阴道出血情况明显较正常组多,精神状态差,皮毛光泽度差,活动度减弱。(2)与模型组相比,中药中剂量组流产率明显较模型组降低(P<0.01),中药高、低剂量组流产率较模型组降低(P<0.05)。与正常组相比,中药中剂量组流产率无差异(P>0.05)。结论:庞氏安胎汤可以降低先兆流产大鼠的流产率,促进胚胎的成长,庞氏安胎汤中剂量保胎效果较西药地屈孕酮效果好。

Objective：To observe the general condition of the rats with threatened abortion,including the mental state,the glossiness of the fur,the activity,the vaginal bleeding,the presence or absence of pregnancy tissue,by adding Pangshi Antai Decoction and western medicine Dydrogesterone.To observe the abortion rate of pregnant rats in each group and the effect of Pongshi Antai Decoction on threatened abortion in rats.Methods：Female SD rats were randomly divided into normal group,model threatened abortion group,western medicine Dydrogesterone group group,low dose of traditional Chinese medicine group,middle dose of traditional Chinese medicine group,and high dose of Chinese medicine group,with 10 rats in each group.After the administration of western medicine and traditional Chinese medicine for 12 weeks,at 8：00 in the morning of 12 days after pregnancy,except for the normal group,the other groups were administrated with Mifepristone to establish the threatened abortion rat model,and the general situation of pregnant rats such as the mental state,the glossiness of the fur,the activity,the vaginal bleeding,the presence or absence of pregnancy tissue.The rats in each group were sacrificed on the thirteenth day of pregnancy.The morphology and size of each group were observed.The number of implantation sites,the number of live births and the number of stillbirths were recorded and the effect of Pongshi Antai Decoction on threatened abortion in rats was observed.Results：（1）The threatened abortion model was successful established：the abortion rate of the model group was significantly higher than that of the normal group（P 〈 0.01）.The vaginal bleeding of the model group of rats was significantly more than that of the normal group,besides the poor mental state,poor skin gloss,and weakened activity.（2）Compared with that of the model group,the abortion rate of the middle dose of traditional Chinese medicine group was significantly lower than that of the model group（P 〈 0.01）.The rate of abortion in the high and low dose of traditional Chinese medicine group was significantly lower than that of the model group（P 〈 0.05）.Compared with that of the normal group,the abortion rate of the middle dose of traditional Chinese medicine group was not significant（P 〉0.05）.Conclusion：Pongshi Antai Decoction can reduce the abortion rate and promote the growth of embryos and the effect of Pongshi Antai Gecoction is significantly better than that of western medicine.