不同处理对梅叶冬青插穗生根的影响

Effects of different treatments on cuttings rooting in Ilex asprella

为提高梅叶冬青扦插育苗的成活率以及扦插苗质量,研究了插穗粗度、基质类型、ABT-1生根粉浓度、植物生长调节剂类型(NAA、IBA)及其浓度、细胞活性成分(二甲亚砜DMSO)和抗氧化剂(抗坏血酸VC、聚乙烯吡咯烷酮PVP)的添加对梅叶冬青扦插育苗的影响。结果表明:插穗粗度、基质种类和生长调节剂浓度对梅叶冬青扦插生根影响显著。插穗粗度以直径0.9~1.6 cm的生根效果最好。黄土、河沙、园土3种基质中,黄土基质生根效果最好。就生长调节剂浓度而言,以低浓度(50 mg/L NAA、50 mg/L IBA、100 mg/L IBA或200 mg/L ABT-1)处理最佳。ABT-1、IBA、NAA的使用均显著提高了梅叶冬青的生根率,但2种生长调节剂IBA和NAA对生根率的影响差异不显著。以黄土为基质,在50 mg/L NAA的基础上添加抗氧化剂VC(抗坏血酸)显著抑制了梅叶冬青的扦插生根,添加抗氧化剂PVP和细胞活性组分DMSO显著促进了梅叶冬青生根。在所有的处理中,50 mg/L NAA+600 mg/L PVP+0.5%DMSO的处理生根率最高,达到48.89%。

In order to improve survival rate of cuttings and quality of cutting seedlings in Ilex asprella, effects of five factors on cuttage propagation were researched, including cuttings diameter, medium type, ABT-1 concentration, type and concentration of plant growth regulator, cell active component （DMSO）, antioxidant （VC and PVP）. The results showed that cuttings diameter, medium type, and concentration of plant growth regulator had significant effects on cottage propagation in I. asprella. The cuttings with 0.9-1.6 cm diameter had the best rooting effect. Loess was more suitable medium for rooting than other media, such as sand and garden soil. In terms of concentration of plant growth regulator, lower concentrations had better effects, for example, 50 mg/L NAA, 50 mg/L IBA, 100 mg/L IBA, or 200 mg/L ABT-I. Application of ABT- 1, IBA and NAA could significantly enhance rooting rate, but effects of IBA and NAA on rooting rate had no significant difference. Using loess as medium, antioxidant VC added in 50 mg/L NAA treatment could significantly inhibit cuttings rooting, and antioxidant PVP and active component DMSO could promote rooting. In the combinations, the treatment of 50 mg/L NAA ＋ 600 mg/L PVP ＋ 0.5% DMSO had the highest rooting rate, which reached 48.89%.