摘要
目的研究不同君药四君子汤对受照人淋巴母细胞AHH-1及小鼠的辐射损伤防护作用,探讨不同君药及不同配比对四君子汤抗辐射活性的影响。方法使用CCK-8试剂盒检测不同终浓度的3种四君子汤作用48 h对AHH-1细胞的毒性作用,以及不同终浓度的四君子汤预处理AHH-1细胞2 h后对经4 Gy^(60)Coγ射线照射的细胞存活率的影响。使用6~8周的BALB/C小鼠120只,用随机数字表法分为阴性对照组、照射对照组以及人参四君子汤(10:9:9:6)组、人参四君子汤(2:2:2:1)组、党参四君子汤(2:2:2:1)组。3.5 Gy^(60)Coγ射线全身单次照射造成小鼠电离辐射损伤模型。照射后3 d和7 d,观察不同君药、不同配比的四君子汤对细胞、辐射损伤模型小鼠的体重、胸腺系数、脾脏系数、微核、脾细胞凋亡等活性指标的影响。结果 250μg/mL的党参四君子汤(2:2:2:1)可显著提高受照AHH-1的存活率(P<0.05);人参四君子汤(2:2:2:1)、党参四君子汤(2:2:2:1)能够有效促进照后3d小鼠体重的恢复(P<0.05);人参四君子汤(10:9:9:6)可显著提高照后7d小鼠的胸腺系数、显著降低照后3d的骨髓嗜多染红细胞微核率及照后3 d、7 d的脾细胞凋亡率。党参四君子汤(2:2:2:1)可显著降低照后3 d、7 d的脾细胞凋亡率及骨髓嗜多染红细胞微核率。人参四君子汤(2:2:2:1)可显著升高照后7d的胸腺、脾脏系数,显著降低照后3 d、7 d的脾细胞凋亡率及骨髓嗜多染红细胞微核率。结论相同配比下,人参作为君药,抗辐射药效优于党参;不同配比下,人参(2:2:2:1)抗辐射药效优于人参(10:9:9:6)。该实验结果验证了四君子汤方中人参作为君药以及药物配比为2:2:2:1的合理性,同时也验证了党参四君子汤及10:9:9:6的药物配比在辐射损伤防治方面也起到一定的作用。
Objective To observe the radiation - protective effect of different types of Sijunzi Decoction on AHH - 1 and BALB/c mice. Methods CCK - 8 Assay Kit was used to detect the survival rate of AHH - 1 cells treated by the extract of dif- ferent types of Sijunzi Decoction for 48h on different dosages. According to the results, the radiation - protective effect of dif- ferent types of Sijunzi Decoction on AHH - 1 single - irradiated by 4.0 Gy ^60Co γ- rays was investigated. One hundred and twenty BALB/c mice aged 6 - 8 weeks were divided by random number table method into normal control group, radiation group, Ginseng Sijunzi Decoction( 10: 9: 9: 6) group, Ginseng Sijunzi Decoction(2: 2: 2:1 ) group, and Codonopsis Sijunzi De- coc.tion ( 2: 2: 2:1 ) group. Radiation - damaged model mice were single - irradiated by 3.5 Gy ^60Co γ- rays, and the extract of different types of Sijunzi Decoction were administered intragastilcally before and after the irradiation. The activity index in- eluding body weight, thymus index, spleen index, bone marrow mieronueleus rate and apoptosis of spleen cells were detected. Results Compared to the radiation group, the survival rate of irradiated AHH - 1 cells were increased by Codonopsis Sijunzi Deeoction( 2: 2: 2:1 ) at the dosage of 250μg/mL( P 〈 0.05 ). The body weight recovery of mice were promoted by Ginseng Si- junzi Decoction(2: 2: 2:1 ) and Codonopsis Sijunzi Decoction( 2: 2: 2:1 ) at 3d after irradiation( P 〈 0.05 ). At 7d after irradiation, the mice thymus index were elevated by Ginseng Sijunzi Decoction( 10: 9: 9: 6) (P 〈0.05), also the increasement of mice bone marrow micronucleus rates were inhibited by Ginseng Sijunzi Decoction( 10: 9: 9: 6)(P 〈0.05 )at 3d after irradiation. Besides, Ginseng Sijunzi Decoction (10: 9:9:6)has the significant effect on reducing the apoptosis of spleen cells at 3d or 7d after irradiation. Codonopsis Sijunzi Decoction(2: 2: 2:1 ) has significant effect on inhibiting the increasement of mice bone marrow micronucleus rates and reducing the apoptosis of spleen cells at 3d or 7d after irradiation. Ginseng Sijunzi Decoc- tion(2: 2: 2:1 )has the same effect with Codonopsis Sijunzi Decoction(2: 2: 2:1 )and it can also raise mice thymus index, spleen index greatly at 7d after irradiation. Conclusion On the same proportion of the principal drug, Ginseng Sijunzi Decoction was more effective. On the other hand, Ginseng Sijunzi Decoction(2: 2: 2:1 )was more effective than Ginseng Sijunzi De- coction( 10: 9: 9: 6). The experiment results indicate the correctness of the following points of view: a, Ginseng is more appro- pilate for the principal drug, b. the proportion of (2: 2: 2:1 )is more effective on the radiation -protective effection, c. Codonopsis Sijunzi Decoction and the proportion of (10: 9: 9: 6)also play a role in the radiation- protective effection.
出处
《中国辐射卫生》
2017年第3期265-269,共5页
Chinese Journal of Radiological Health
关键词
四君子汤
人淋巴母细胞
辐射防护
人参
党参
Sijunzi Decoction
AHH - 1
Radiation - Protective
Ginseng
Codonopsis