不同栽培年限亳芍多糖与微量元素动态积累规律研究

The dynamic accumulation of polysaccharide and trace elements from Bozhou Peony Paeonia lactiflora with different harvesting ages

对采自安徽亳州地区人工栽培1~7年亳芍样品,水提醇沉法提取多糖,蒽酮-硫酸法和原子吸收光谱法(FAAS)分别测定样品中多糖及微量元素含量,探讨多糖、微量元素在亳芍中的动态积累规律,为亳芍采收、品质评价与规范化栽培提供依据。结果表明,栽培年限对亳芍多糖和微量元素的积累有显著影响:株龄1~7年的亳芍,多糖的含量分别为(2.06±0.12)%、(3.59±0.17)%、(4.66±0.26)%、(3.14±0.19)%、(2.44±0.03)%、(2.49±0.12)%和(2.32±0.20)%;株龄1~7年的亳芍铜元素、铅元素、铬元素、镁元素、钙元素、钠元素、锌元素和钡元素的平均质量分数分别为(3.28±0.63)、(3.16±0.63)、(1.69±0.09)、(300.17±13.64)、(1476.66±57.49)、(71.88±2.26)、(8.58±0.56)和(369.51±26.68)mg/kg(DW);3种有害微量元素含量未超过5.0 mg/kg(DW)限量指标,均在规定限度范围内。上述结果显示,株龄3年亳芍多糖含量最高,亳芍中含丰富的人体必需微量元素,重金属限量指标整体上是安全的,微量元素的含量与多糖的含量存在相关性。

The contents of the total polysaccharide and the 8 trace elements of 1-7 years-old Bozhou Peony were detected by anthracenone-concentrated-sulfuric-acid colorimetry and AAS,respectively,to explore the dynamic accumulation of polysaccharides and 8trace elements in Bozhou Peony Paeonia lactiflora,and to provide scientific basis for the optimum harvesting age,quality evaluation and standardized cultivation of Bozhou Peony. The results indicated that cultivating ages would significantly impact the accumulation of polysaccharide and trace elements. The average contents of polysaccharides of Bozhou Peony（ from 1 to 7 years） were：（ 2. 06 ±0. 12） %,（ 3. 59 ± 0. 17） %,（ 4. 66 ± 0. 26） %,（ 3. 14 ± 0. 19） %,（ 2. 44 ± 0. 03） %,（ 2. 49 ± 0. 12） % and（ 2. 94 ± 0. 20） %;the mass fractions of Cu,Pb,Cr,Mg,Ca,Na,Zn and Ba were（ 4. 14 ± 0. 39）,（ 5. 02 ± 0. 87）,（ 1. 69 ± 0. 07）,（ 300. 17 ±13. 64）,（ 1476. 66 ± 57. 49）,（ 71. 88 ± 2. 26）,（ 8. 58 ± 0. 56） and（ 369. 51 ± 26. 68） mg/kg（ DW）,respectively,and all the mental elements were in limits except Pd content in the samples exceeded the standard limit 0. 89 mg kg（ DW）. Our experimental suggested that the Bozhou Peony cultivated for 3 years obtained the highest polysaccharide. There were rich essential mental elements in Bozhou Peony and the contents of heavy mental elements were lower than the limited. There might exist correlation between the trace elements contents and the accumulation of polysaccharide in Bozhou Peony.