EB病毒抗体及DNA联合检测对婴幼儿传染性单核细胞增多症的诊断价值

Value of Combined Detection of EB Virus Antibody and Its DNA in Diagnosis of Infectious Mononucleosis Syndrome in Infants and Young Children

目的分析EB病毒(EBV)抗体及EBV-DNA联合检测在婴幼儿传染性单核细胞增多症(infectious mononuleosis,IM)中的诊断价值。方法选择我院儿科2014年1月—2016年5月诊断的120例IM患儿,分别于入院第1、2、3、4周采用酶联免疫吸附法检测EBV-Ig M,采用荧光定量法检测EBV-DNA载量,以及EBV-Ig M联合EBVDNA检测,比较3种检测方法的阳性率。结果入院第1、2、3、4周EBV-Ig M检测及联合检测阳性率均高于EBVDNA检测阳性率,且第1、2周联合检测阳性率亦高于EBV-Ig M检测阳性率,差异均有统计学意义(P<0.05或P<0.01);入院第3、4周联合检测与EBV-Ig M检测阳性率比较差异无统计学意义(P>0.05)。结论 EBV-Ig M检测对于婴幼儿IM早期诊断更为敏感,而早期EBV-Ig M联合EBV-DNA检测可进一步提高诊断率。

Objective To analyze value of combined detection of epstein-barr virus （ EBV） antibodies and EBV-DNA in diagnosis of infectious mononucleosis syndrome （ IMS） in infants and young children. Methods A total of 120 infants and young children with IMS admitted during January 2014 and May 2016 were recruited in this study. In the 1st, 2nd, 3rd and 4th weeks after admission, EBV-immunoglobulin M （ IgM） levels were detected using enzyme linked immunosorbent assay;EBV-DNA values was detected using fluorescent quantitation;combined detection of EBV-IgM and EBV-DNA were also performed. Positive rates of 3 methods were compared. Results Positive rates in the 1st, 2nd, 3rd and 4th weeks after admission by EBV-IgM and combined detections were significantly higher than those of EBV-DNA, and the positive rates of combined detection in the 1st and 2nd weeks after admission were significantly higher than those of EBV-IgM detection （P〈0. 05 or P〈0. 01）. There were no statistically significant differences in the 3 rd and 4 th weeks after admission between combined detection and EBV-IgM detection （ P〉0. 05 ） . Conclusion EBV-IgM detection is more sensitive for early diagnosis of infants and young children with IMS, and early combined detection of EBV-IgM and EBV-DNA can further increase diagnostic rate.