淫羊藿苷对大鼠正畸牙移动过程中牙槽骨改建的影响

Effect of icraiin on turnover of alveolar bone during orthodontic tooth movement in rats

目的:研究淫羊藿苷对大鼠正畸牙移动过程中牙槽骨改建的作用机制。方法:48只雄性Sprague-Dawley(SD)大鼠,建立正畸牙移动模型,随机分为对照组和淫羊藿苷组,每组分为4个亚组,每个亚组6只。实验组以灌胃方式给予大鼠淫羊藿苷20 mg/(kg·d),对照组给予等体积溶剂。各组大鼠在7、14、21、28 d处死,分离出上颌骨作为标本,测量上颌第一磨牙近中移动距离。所有标本做脱钙处理,制成上颌牙周组织切片,分别采用H-E染色、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色观察移动牙周围牙周膜及牙槽骨的改建情况。采用SPSS16.0软件包对数据进行统计学分析。结果:淫羊藿苷组牙移动距离显著大于对照组(P<0.05)。光镜下显示,淫羊藿苷组压力侧破骨细胞数目在第7天时达到高峰,显著高于对照组;在第14天时与对照组持平,至28 d时低于对照组。结论:淫羊藿苷通过促进牙槽骨的改建而加速正畸牙移动。

PURPOSE： To observe the effect of icariin on turnover of paradentium as well as the mechanism of effects on osteoclast during orthodontic tooth movement in rats. METHODS： Forty-right SD rats were used to establish animal models of OTM and divided into two groups randomly： the control group and the experimental group. Rats in the experimental group were given 20 mg/（kg·d） icariin by intragastric administration, while rats in the control group received solvent with the same volume. The rats were sacrificed in batch on the 7th, 14th, 21st and 28th day after orthodontic treatment. The amount of tooth movement was measured, and histomorphometric analysis based on slices from periodontium adjacent to the maxillary first molars was used to observe new bone formation, bone resorption and quantify osteoclasts. Statistical analysis was performed by using SPSS 16.0 software package. RESULTS＂ The movement distance of the first molar was significantly larger in the icariin group than that in the control group （P〈0.05）. In the icariin group, the number of osteoclasts was significantly higher than the control group at the 7th day and then demonstrated a steady decrease. CONCLUSIONS： Icariin could accelerate OTM in rats through promoting bone turnover of alveolar bone.