MiR-486通过调控Sirt1调节TF-1细胞的糖代谢

MiR-486 Regulates the Glycometabolism of Hematopoietic Cells by Targeting Sirt1

目的:探索低氧条件下造血细胞miR-486表达变化及miR-486对造血细胞糖代谢的调控作用及机制。方法:利用实时定量PCR(quantitative real-time PCR,qRT-PCR)方法检测低氧条件下TF-1细胞miR-486及Sirt1的表达水平。用慢病毒技术将miR-486过表达或沉默后检测Sirt1、葡萄糖转运因子1(glucose transporter 1,Glut1)和葡萄糖转运因子4(glucose transporter 4,Glut4)的表达水平。另外,将Sirt1沉默后分别用qRT-PCR及Western blot检测Sirt1的表达,并用qRT-PCR检测Glut1和Glut4的表达水平。结果:低氧促进miR-486的表达并抑制Sirt1的表达;过表达miR-486导致Sirt1的表达下调,而沉默miR-486可以促进Sirt1的表达;过表达miR-486上调Glut1和Glut4的表达,而沉默miR-486则抑制Glut1和Glut4的表达;沉默Sirt1可以促进Glut1和Glut4的表达。结论:MiR-486可以通过影响Sirt1调节TF-1细胞的糖代谢。

Objective： To investigate the effect and mechanism of miR-486 on glycometabolism of hematopoietic cells. Methods： qRT-PCR was applied to detect the expression of miR-486 or Sirt1 on TF-1 cells under hypoxia.Lentivirus was used to mediate the overexpression or inhibition of miR-486 on TF-1 cells and qRT-PCR was used to detect the expressions of Sirt1,glucose transporter 1（Glut1） and glucose transporter 4（Glut4）. Lentivirus-mediated Sirt1-shRNA transduction was used to knockdown Sirt1 expression which was detected by qRT-PCR and Western blot.The expressions of Glut1 and Glut4 were determined by qRT-PCR. Results： Hypoxia promoted the expression of miR-486 and inhibited the expression of Sirt1. MiR-486 overexpression could inhibit the expression of Sirt1 and promote the expressions of Glut1 and Glut4,whereas miR-486 silencing upregulated the sirt1 expression and inhibited the expressions of Glut1 and Glut4. And inhibition of Sirt1 expression increased the expressions of Glut1 and Glut4. Conclusion： MiR-486 can regulate the glycometabolism of hematopoietic cells by targeting Sirt1.