UPLC测定全缘叶绿绒蒿花中3种黄酮类成分

Determination of Three Flavonoids in Flower of Meconopsis integrifolia by UPLC

目的：采用UPLC测定11批全缘叶绿绒蒿花中槲皮素-3-O-β-D-葡萄糖-（1→6）-β-D-葡萄糖苷（M1）,槲皮素-3-O-[2-O-乙酰基-β-D-葡萄糖-（1→6）-β-D-葡萄糖苷]（M2）和异槲皮苷（M3）的含量。方法：采用超声提取法,提取溶剂为体积分数90%甲醇,提取时间为45 min,对全缘叶绿绒蒿样品进行处理。色谱柱为ACQUITY UPLC HSS T3（2.1 mm×100 mm,1.8μm）,流速300μL·min-（-1）,柱温35℃,检测波长255 nm,以乙腈-0.1%冰乙酸水（16∶84）为流动相,洗脱时间10 min。结果：在11批全缘叶绿绒蒿花样品中,不同产地、不同采集时间的样品所含有的3种黄酮类化学成分含量各不相同,但总体上两种槲皮素葡萄糖苷含量较高,而异槲皮苷的含量较低;马尔康县梦笔山所采样品黄酮苷总含量高于其他采集地,5月和6月采集的样品黄酮苷总含量高于7月。结论：全缘叶绿绒蒿花中所含主要化学成分含量随产地、采收月份不同而有所差异。该方法准确且具有较高灵敏度,可较准确反应全缘叶绿绒蒿花中化学成分的含量,可为全缘叶绿绒蒿花的质量评价和质量标准提高提供依据。

Objective： To quantitatively analyze quercitrin-3-O-β-D-glucose-（ 1 →6）-β-D-glucoside（ M1）,quercitrin-3-O-[2-O-acetyl-β-D-glucose-（ 1→6）-β-D-glucoside]（ M2）,and isoquercitrin（ M3） in11 batches of flowers of Meconopsis integrifolia by ultra performance liquid chromatography（ UPLC）. Method： The ultrasonic extraction method was used with processing time of 45 min,and the extraction solvent was 90% methanol aqueous solution. ACQUITY UPLC HSS T3 chromatographic column was used at the column temperature of 35 ℃,flow rate of 300 μL·min-1 and the detection wavelength of 255 nm,with acetonitrile-0. 1% glacial acetic acid solution（ 16 ∶ 84） as the mobile phase for isocratic elution for 10 min. Result： The contents of 3 flavonoids in 11 batches of flowers of M. integrifolia in different regions and at different acquisition time were different. Generally,the contents of quercitrin were higher than the content of isoquercitrin; the total content of flavonoid glycosides in Mengbi mountain,Maerkang county was higher than that in the other regions; and the content of flavonoid glycosides from the samples collected in May and June were higher than those collected in July. Conclusion： The contents of main components in 11 batches of flowers of M. integrifolia were relevant to their origins and collection time. The method in this study could reflect the exact contents of compositions,providing reference for quality evaluation and quality standard improvement of M. integrifolia.