不同促渗剂对蒿甲醚软膏体外透皮吸收的影响

Effects of Different Enhancers on in Vitro Transdermal Absorption of Artemether Ointment

目的:考察促渗剂氮酮、冰片、薄荷油对蒿甲醚软膏体外透皮吸收的影响。方法:使用智能透皮试验仪,选择小鼠腹皮为渗透屏障,对含3种促渗剂及其复配体系的软膏进行体外经皮扩散实验,HPLC测定蒿甲醚的累积透过量和经皮扩散速率。结果:2%氮酮、2%冰片、2%薄荷油,2%氮酮+2%冰片、2%氮酮+2%薄荷油均可以不同程度地促进蒿甲醚软膏的透皮吸收,其促渗效果顺序为2%氮酮+2%薄荷油>2%氮酮+2%冰片>2%薄荷油>2%冰片>2%氮酮。结论:薄荷油单用和复配使用对蒿甲醚的体外透皮吸收均有良好的促进作用,复配使用促透效果更佳。

Objective： To investigate the effects of Azone,borneol and peppermint oil on the in vitro transdermal absorption of artemether ointment. Methods： In an intelligent transdermal test instrument,the cumulative permeating amounts and percutaneous rates of artemether containing different enhancers was determined by HPLC,using the abdominal skin of mice as penetration barrier. Results： 2% Azone,2% borneol,2% peppermint oil,2% Azone ＋ 2% borneol and 2% Azone ＋ 2% peppermint oil were found to have different degree penetration enhancing effect,followed successively by 2% Azone ＋ 2% peppermint oil 2% Azone＋ 2% borneol 2% peppermint oil 2% borneol 2% Azone. Conclusion： Using peppermint oil alone or with Azone promotes transdermal absorption of artemether significantly and the combination of peppermint oil with Azone has the best penetration enhancing effect.