中医不同治法对绝经后骨质疏松症大鼠骨组织Hedgehog信号通路mRNA和蛋白表达的影响

Effects of different treatment laws of TCM on the mRNA and protein expression of Hedgehog in bone tissues of osteoporotic rats

目的观察去卵巢骨质疏松症大鼠模型骨组织Hedgehog信号通路中SHH、GLI1 mRNA和蛋白活性变化,探究中药防治骨质疏松症病机的分子生物学机制。方法实验采用切除雌性SD大鼠双侧卵巢的方式建立骨质疏松症模型,运用补肾填精中药复方、活血化瘀中药复方、补肾活血中药复方对模型大鼠进行干预12周,用骨疏康作为阳性药物对照组,以及正常组和模型组。ELISA法检测各组骨组织SHH、GLI1蛋白含量;RT-PCR检测各组SHH、GLI1 mRNA相对表达量。结果骨组织SHH、GLI1蛋白与正常组相比模型组含量明显减少(P<0.01),与模型组相比较,各用药组均明显增加(P<0.01)。结论骨质疏松症的发生机制与Hedgehog信号传导通路SHH、GLI1 mRNA和蛋白活性下降有关;补肾填精中药复方、活血化瘀中药复方、补肾活血中药复方通过激活Hedgehog信号传导通路中的SHH、GLI1,以促进骨形成,抑制骨吸收,起到防止骨质疏松症的作用。

Objective To observe the mRNA and protein expression of SHH and GLI11 in bone tissues of rats with osteoporosis, and to discuss the molecular biology mechanism of using Chinese herbs compound in the prevention and treatment of osteoporosis. Methods We established osteoporosis rat model by bilateral ovariectomy of female rats. Then the osteoporotic rats were treated with the experimental drugs of Chinese herb compound groups including invigorating the kidney and filling in the essence, promoting blood circulation by removing blood stasis, and invigorating the kidney and promoting blood circulation for 8 weeks. GUSHUKANG was taken as the positive control group. Normal control and model control were set for comparison. We used the ELISA and RT-PCR methods to investigate the mRNA and protein expression of SHH and Gli11 in bone tissues. Results The mRNA and protein expression of SHH and Gli11 in bone tissues： model control group had a decrease compared with the normal group （P〈0.01）. Different levels of increase were detected in all treatment groups compared with the model control group （P〈0.01）. Conclusion The mechanism of osteoporosis was related to decreased protein expression of SHH and Gli11. The Chinese herb compounds can promote osteoblast differentiation, and inhibit osteoclast activity, thus have the effect of preventing osteoporosis by activating the Notch signaling pathways.