萝卜硫素对免疫抑制小鼠免疫功能的影响

The immunoregulation effect of sulforaphane in immunosuppressed mice

目的观察萝卜硫素(SF)对环磷酰胺(Cy)所致免疫抑制小鼠的迟发性超敏反应及IL-10及TNF-α表达的影响,探讨SF对机体免疫系统的调节机制。方法 ICR小鼠随机分4组:正常对照组、Cy模型组、SF高剂量组(50mg/kg)、SF低剂量组(25mg/kg),每组10只。采取灌胃给药法。实验10d后,检测小鼠胸腺(脾脏)指数、小鼠血清溶血素的浓度;DNFB致敏观察小鼠迟发型超敏反应(DTH反应);ELISA测定外周血脾脏细胞白细胞介素-10(IL-10)、肿瘤坏死因子-a(TNF-α)的表达水平;RT-PCR检测小鼠细胞IL-10、TNF-αmRNA的表达水平。结果相对于Cy模型组,SF高、低剂量组胸腺指数、脾脏指数以及小鼠耳廓的肿胀度增大,血清溶血素水平升高,外周血TNF-α的表达升高,IL-10的表达降低,差异具有统计学意义(P均<0.05);与Cy模型组相比,SF高、低剂量组的脾脏细胞TNF-αmRNA表达升高,IL-10mRNA表达降低,差异具有统计学意义(P均<0.05)。结论 SF对Cy所致的免疫低下小鼠有较强的免疫保护作用,可能通过促进TNF-α的分泌上调免疫功能,或通过抑制IL-10的表达降低机体的免疫负调节。

Objective To study the immunoregulation effect of Sulforaphane by observing its effect on IL-10,TNF-α expressions and the delayed type hypersensitivity( DTH) in cyclophosphamide induced immunosuppressed mice. Methods The ICR mice were divided into four groups randomly( 10 mice in each group) : control group,cyclophosphamide( Cy) group,Sulforaphane high-dose group( 50 mg/kg) and Sulforaphane low-dose group( 25 mg/kg). The intragastric administration was used,and the thymus and spleen index,the content of serum hemolysin,the DTH reaction,the expression of Interleukin( IL-10) and Tumor necrosis factor( TNF-α) was analyzed after 10 days. Results Compared with Cy model group,Sulforaphane high-dose and low-dose groups increased the number of spleen index,thymus index,hemolysin OD value and the TNF-α concentration. the expression of TNF-α mRNA,and decreased the IL-10 concentration,the expression of IL-10 mRNA( P < 0. 05). Conclusion Sulforaphane can notably improve the immune function in immune-suppressive mice by increasing the expression of TNF-α or decreasing the expression of IL-0.