摘要
目的探讨血浆游离DNA水平在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的诊断价值。方法选取陕西省人民医院健康中心120例健康对照者,并提取收治呼吸内科的116例非小细胞肺癌患者的,采用荧光定量PCR方法检测血浆游离DNA水平。结果非小细胞肺癌组患者的血浆游离DNA水平为200.70±88.54 ng/ml,健康对照组的血浆游离DNA水平为18.65±6.30 ng/ml,组间比较差异有统计学意义(P<0.01);Ⅰ/Ⅱ期非小细胞肺癌患者的血浆游离DNA水平为172.75±72.87 ng/ml,Ⅲ/Ⅳ期非小细胞肺癌血浆游离DNA水平为221.88±93.86 ng/ml,组间比较差异有统计学意义(P<0.05)。血浆游离DNA水平与患者的年龄、性别、吸烟状况及组织学类型无明显相关性(P>0.05)。结论血浆游离DNA是一种新发现的肿瘤标志物,可作为非小细胞肺癌早期诊断和临床分期的有效工具。
Objective To evaluate the value of plasma free DNA in the diagnosis of non-small cell lung cancer( NSCLC). Methods Plasma was collected from 116 patients with non-small cell lung cancer admitted to respiratory department, and 120 healthy subjects from Medical examination center, Shanxi Provincial People' s Hospital. Plasma free DNA was extracted and detected by fluorescence quantitative polymerase chain reaction,PCR. Results The plasma free DNA level of healthy people was( 18. 65 ± 6. 30)ng/ml,and the plasma free DNA level in patients with non-small cell lung cancer was( 200.70±88.54) ng/ml,the difference was statistically significant( P<0.01). The plasma free DNA content of stage Ⅰ/Ⅱ non-small cell lung cancer was( 172.75± 72. 87) ng/ml,and the plasma circulating DNA content in stage Ⅲ/Ⅳ non-small cell lung cancer was( 221.88 ± 93. 86) ng/ml,( P < 0. 05). There was no significant correlation between sex,age,smoking status and histological type( P > 0. 05). Conclusion Plasma circulating DNA is a potentially valuable biomarker that may be an effective tool for early diagnosis and clinical staging of non-small cell lung cancer.
出处
《中华肺部疾病杂志（电子版）》
CAS
2017年第6期686-689,共4页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
陕西省科技厅2013年科技惠民专项(2013K14-02-09)
