电针肋间神经干预大鼠乳腺增生病的实验研究

Electroacupuncture at intercostal nerve for mammary gland hyperplasia in rats

目的:探讨电针治疗乳腺增生病疗效的发挥与肋间神经调节途径是否具有一定的相关性。方法:选用雌性SD大鼠50只,随机分为空白组(A组)、模型组(B组)、电针组(C组)和肋间神经切断后干预组(D组),A、B组各12只,C、D组各13只。B、C、D组均制备乳腺增生模型,造模成功后,手术将D组大鼠左侧第7肋间神经切断。C、D组选背组穴:"天宗""肝俞""肾俞";胸组穴:"屋翳""合谷""膻中",进行电针干预,每次留针20 min,每日1次,两组穴位交替选用,5 d为一疗程,疗程间休息2 d,共干预20次。干预结束后,观察各组大鼠乳头高度和直径、血清雌二醇(E2)、孕激素(P)及乳腺组织中雌激素受体α(ERα)和孕激素受体(PR)的含量变化。结果:(1)大鼠乳头高度和直径:干预后,与B组比较,C组乳头高度和直径明显较小(均P<0.05);与C组左侧乳头比较,D组左侧乳头高度和直径较大(均P<0.05)。(2)血清E_2、P:干预后,与B组比较,C、D组血清E_2、E_2/P含量下调,P含量上调(均P<0.05);与C组比较,D组血清E_2、E_2/P含量上调,P含量下调(均P<0.05)。(3)乳腺组织中ERα、PR:与B组比较,C组ERα含量下调、PR含量上调(均P<0.05);与C组比较,D组ERα含量上调、PR含量下调(均P<0.05)。结论:电针干预乳腺增生的疗效机制可能与肋间神经通路具有密切关系,可能是通过调节血清E_2、P含量及乳腺组织中ERα、PR蛋白含量实现的。

Objective To explore the correlation between efficacy of electroacupuncture （EA） on mammary gland hyperplasia （MGH） and the regulatory pathway of intercostal nerve. Methods Fifty female SD rats were randomly divided into a blank group （group A, 12 rats）, a model group （group B, 12 rats）, an EA group （group C, 13 rats） and an intercostal nerve transection group （group D, 13 rats）. The rats in the group B, group C and group D were prepared into MGH model;after model was successfully prepared, the 7th intercostal nerve was cut off in the group D. EA was applied at back acupoints including bilateral ＂Tianzong＂ （SI 11）, ＂Ganshu＂ （BL 18） and ＂Shenshu＂ （BL 23） as well as chest acupoints including bilateral ＂Wuyi＂（ST 15）,＂Hegu＂（LI 4） and＂Danzhong＂（CV 17） in the group C and D. The two groups of acupoints were selected alternately. EA was given for 20 min, once a day;5-day treatment was taken as one course;there was an interval of 2 days between course;totally 20 treatments were given. After treatment, the height and diameter of papilla were observed;the contents of serum estradiol （E2） and progestin （P）, the expression of estrogen receptor α （ERα） and progestrone receptor （PR） in mammary gland were measured. Results （1） The height and diameter of papilla：after treatment, the height and diameter of papilla in the group C were significantly smaller than those in the group B （both P〈0.05）;the height and diameter of left-side papilla in the group D were significantly bigger than those in the group C （both P〈0.05）. （2） Serum E2 and P：after treatment, compared with the group B, the contents of E2 and E2/P were reduced and the content of P was increased in the group C and group D （all P〈0.05）. Compared with the group C, the contents of E2 and E2/P were increased and the content of P was reduced in the group D （all P〈0.05）. （3） ERα and PR in mammary gland： compared with the group B, the content of ERαwas decreased and the content of PR was increased in the group C （both P〈0.05）. Compared with the group C, the content of ERαwas increased and the content of PR was decreased in the group D （（both P〈0.05）. Conclusion The efficacy mechanism of EA for MGH is likely to be related with the pathway of intercostal nerve;the mechanism may be acupuncture regulating the contents of serum E2 and P as well as contents of ERα and PR in mammary gland.