间歇有氧运动通过上调高血压肾病大鼠肾上腺髓质素降低血压机制的研究

Intermittent aerobic exercise decreases blood pressure of hypertensive nephropathy rat by up-regulated expression of adrenomedullin

目的:研究间歇有氧运动(IAE)对高血压肾病大鼠(HNR)肾脏肾上腺髓质素(ADM)的影响,探讨运动降低血压的肾性因素及其机制。方法:HNR 32只,分为间歇有氧运动组(IAE组),对照组(C组)。IAE组进行8w的间歇跑台有氧运动。采用尾压法测血压,酶联免疫吸附法测运动前后血清中ADM的含量,免疫组织化学法显示HNR肾脏ADM的表达,免疫印记法检测肾脏ADM的特异性受体RAMP2和CRLR的定表达量。结果:IAE组血压明显低于C组(P<0.05);IAE组血清中ADM的含量明显高于C组(P<0.05);肾脏ADM表达明显强于C组;肾脏RAMP2和CRLR定表达量均明显高于C组(P<0.05)。结论:间歇有氧运动使HNR的血压下降,其降血压的作用机制可能与运动导致的血液中ADM升高,肾脏ADM表达增强及其特异性受体上调有关。

Objective： To probe the effect of intermittent aerobic exercise on adrenomedullin secretion of kidney in hypertensive nephropathy rat （HNR）, and investigate the renal mechanism for exercise in the prevention and treatment of hypertension. Method： Thirty-two HNRs were divided into two groups： intermittent exercise group （Group IAE） and control group （Group C）. The IAE group performed intermittent treadmill exercise for 8 weeks and their blood pressure was measured before and after exercise by tail pressure of HNR, and serum ADM was detected by enzyme linked immunosorbent assay. Adrenomedullin （ADM） was expressed in kidney by immunohistochemistry. Quantification of specific receptor RAMP2 and CRLR in kidney ADM was detected by immunoblotting. Result： The blood pressure in the Group IAE was significantly lower than that in the Group C（P＜0.05）. The expression of ADM of kidney and serum in the Group IAE was obviously higher than that in the Group C. The quantity of RAMP2 and CRLR in kidney was obviously increased（P＜0.05）. Conclusion： Intermittent aerobic exercise increases serum ADM of HNR with the up-regulated expression of ADM in kidney and their specific receptor CRLR and RAMP2. The increase of serum ADM induced by intermittent aerobic exercise may be related to the change of ADM and the regulation of its specific receptor which may be one of the mechanisms for intermittent aerobic exercise in the prevention and treatment of hypertension.