阿托伐他汀对K562细胞的抗增殖作用及凋亡作用的研究

Antiproliferative and apoptosis effect of atorvastatin on K562 cells

目的观察阿托伐他汀对K562细胞增殖及凋亡的影响,并探讨其作用机制。方法不同浓度的阿托伐他汀处理K562细胞,CCK-8法检测细胞增殖;AnnexinV-FITC/PI双染法检测细胞凋亡;流式细胞仪检测细胞周期;比色法检测半胱氨酸蛋白水解酶(caspase)-3、-8、-9的活性;qRT-PCR检测Bcl-2、程序性死亡因子(PDCD5)mRNA的表达。结果阿托伐他汀至浓度和时间依赖性地抑制K562细胞增殖(P<0.05)并诱导细胞凋亡(P<0.01);阿托伐他汀作用K562细胞后,G0/G1期细胞百分比增加(P<0.01),S期细胞百分比下降(P<0.01),且呈浓度依赖性(P<0.01);阿托伐他汀以浓度依赖性活化caspase-3、-8、-9(P<0.01)、下调Bcl-2mRNA表达和上调PDCD5mRNA的表达(P<0.01)。结论阿托伐他汀可抑制K562细胞增殖并诱导其凋亡。

Objective To explore the effect of atorvastatin on the proliferation and apoptosis of K562 cells andto investigate its mechanisms. Methods The cells were treated by different concentrations of atorvastatin. The CCK-8 assay was employed to detect the cell proliferation. The cell apoptosis was detected by AnnexinV-FITC/PI dual staining; the flow cytometry was used to detect the Cellular cycle; the activities of caspase-3 ,-8,-9 were detected by the colorimetric method;qRT-PCR was employed to measure the mRNA expression levels of Bcl-2 and PDCD5 in K562 cells. Results ：Atorvastatin could inhibit the proliferation of K562 cells in a time- and close-dependent manner（P〈0.05） ;and induced the apoptosis of K562 cells,the percentage of Go/G1 phase cells was increased after atorvastatin treating k562 cells（P〈0.01）, while the percentage of S phase Cells was decreased（P〈0.01）,moreover which showing the concentration dependence（P〈0.01）;atorvastatin activated the caspase-3,-8,-9（P〈0.01）;down-regulated Bcl-2 mRNA expression and up-regulated PDCD5 mRNA expression in a concentration dependence（P〈0.01）. Conclusion Atorvastatin can inhibit the proliferation and induce apoptosis in K562 cells.