慢性乙肝患者血清TGF-β1含量检测及与肝纤维化、Th1/Th2免疫水平的相关关系

The correlation of serum TGF-β1 content with liver fibrosis and Th1/Th2 immune levels in patients with chronic hepatitis b

目的:探讨慢性乙肝患者血清转化生长因子β1(TGF-β1)含量检测及其与肝纤维化、Th1/Th2免疫水平的相关关系。方法:2015年2月~2017年8月在本院确诊为慢性乙型肝炎的患者178例作为慢性乙肝组,同期在本院进行体检的健康志愿者100例作为正常对照组。对比两组研究对象血清中TGF-β1、肝纤维化指标、Th1/Th2细胞因子含量的差异,采用Pearson检验评估慢性乙肝患者血清TGF-β1含量与病情严重程度的相关关系。结果:慢性乙肝组血清中TGF-β1的含量高于正常对照组(P<0.05);血清中肝纤维化指标Ⅳ型胶原(CⅣ)、前胶原蛋白Ⅲ(PC-Ⅲ)、透明质酸(HA)、层黏连蛋白(LN)的含量高于正常对照组(P<0.05);血清中Th1细胞因子干扰素γ(IFN-γ)、白介素(IL)-12的含量低于正常对照组(P<0.05),Th2细胞因子IL-4、IL-13的含量高于正常对照组(P<0.05)。Pearson检验发现,慢性乙肝患者血清TGF-β1含量与肝纤维化程度、Th1/Th2免疫失衡程度均呈正相关(P<0.05)。结论:慢性乙肝患者存在血清TGF-β1异常高表达,与肝纤维化及免疫失衡程度直接相关。

Objective:To investigate the correlation of serum TGF-β1 content with liver fibrosis and Th1/Th2 immune levels in patients with chronic hepatitis b.Methods:A total of 178 patients who were diagnosed with chronic hepatitis b in our hospital between February 2015 and August 2017 were selected as chronic hepatitis b group,and 100 healthy volunteers who received physical examination in our hospital during the same period were selected as normal control group.The differences in serum levels of TGF-β1,liver fibrosis indexes and Th1/Th2 cytokines were compared between the two groups,and the Pearson test was adopted to evaluate the correlation between serum TGF-β1 content and disease severity in patients with chronic hepatitis b.Results:Serum TGF-β1 level of chronic hepatitis b group was higher than that of normal control group(P<0.05);serum liver fibrosis indexes CⅣ,PC-Ⅲ,HA and LN levels were higher than those of normal control group(P<0.05);serum Th1 cytokines IFN-γand IL-12 levels were lower than those of normal control group(P<0.05)whereas Th2 cytokines IL-4 and IL-13 levels were higher than those of normal control group(P<0.05).Pearson test showed that the serum TGF-β1 level in patients with chronic hepatitis b was positively correlated with the degree of liver fibrosis and Th1/Th2 immune imbalance(P<0.05).Conclusions:Serum TGF-β1 expression is abnormally high in patients with chronic hepatitis b and directly correlated with the degree of liver fibrosis and immune imbalance.