摘要
目的研究大鼠腹腔注射丙戊酸钠后游离丙戊酸在大鼠血液和脑内的药动学。方法采用血-脑双位点微透析技术结合柱前衍生化高效液相色谱法,测定给药后240 min内各时间段大鼠血液透析样品和脑部海马区细胞外液透析样品中丙戊酸的浓度。数据采用DAS 3.0软件计算药代动力学参数。结果大鼠海马区细胞外液中游离丙戊酸达峰时间显著滞后于其在血液中的达峰时间,海马区细胞外液中游离丙戊酸的C_(max)和AUC_(0~∞)均明显低于血液中的响应值。结论本文建立的微透析技术结合柱前衍生化HPLC法可以成功用于丙戊酸在大鼠血-脑双位点的药动学研究。
Objective To investigate the pharmacokinetics of free valproate in the brain tissues and blood of rats after intraperitoneal injection of sodium valproate. Methods Blood-brain microdialysis and HPLC following precolumn derivatization were used to determine the concentration of valproate in microdialysis dialysate of blood and extracellular fluids of hippocampus 240 min after a single intraperitoneal injection in the rats. Pharmacokinetic parameters were calculated with DAS 3.0 software. Results tmax of free valproate in the extracellular fluids of the hippocampus lagged behind that in blood. Cmax and AUC0-∞ in the extracellular fluids of the hippocampus were lower than those in the blood. Conclusion Microdialysis with HPLC following precolumn derivatization established in this study can be utilized to successfully determine the pharmacokinetics of valproate in rat brain tissues and blood.
出处
《中南药学》
CAS
2018年第2期148-151,共4页
Central South Pharmacy
基金
上海市卫生和计划生育委员会基金项目(编号:20144Y0233)
