正畸牙移动中牙周组织VEGF的表达及在骨改建中的作用机制探讨

Expression of vascular endothelial growth factor in periodontal tissues during orthodontic tooth movement and its role in bone remodeling

目的 :通过检测血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)在大鼠正畸牙移动牙周组织骨改建过程中VEGF表达的变化,探讨VEGF在正畸牙移动中的作用机制。方法:首先建立磨牙移动的大鼠实验模型,并将实验组的30只大鼠分为5组,每组6只,依次在第2、4、7、14天及20天后处死,去除磨牙,制作牙周组织切片。使用H-E染色方法,观察牙周组织的形态变化。利用免疫组织化学染色对VEGF的表达进行半定量分析。采用SPSS21.0软件包对数据进行统计学分析。结果 :正常大鼠牙周组织中VEGF表达很弱,甚至不表达。在牙出现松动的第2天,VEGF在牙周组织中表达增强;第7~14天表达逐渐增强,第14天达到高峰;第20天以后VEGF表达略有下降,但仍高于对照组。血管内皮细胞、成纤维细胞、成骨细胞、破骨细胞胞质是VEGF的主要表达部位。结论:VEGF参与正畸牙移动过程中牙周组织的早期改建过程,并可能在其中发挥重要作用。

PURPOSE： To investigate the expression of VEGF in periodontal tissues and its role in bone remodeling during orthodontic tooth movement in rats. METHODS： Rat model of tooth movement was established, and 30 rats in the experimental group were divided into 5 groups, 6 rats in each group. At 2 days, 4 days, 7 days, 14 days and 20 days, the rats were sacrificed to make periodontal tissue slice. The morphological changes of periodontal tissue were observed under H-E staining, and the expression of VEGF was semi-quantitatively analyzed by immunohistochemistry. The data were analyzed using SPSS21.0 software package. RESULTS： Expression of VEGF in normal rat periodontal tissues was very weak or without positive expression; 2 days after tooth movement, VEGF expression in periodontal tissues began to increase from seventh to fourteenth days; on the fourteenth day, the expression reached a peak; after twentieth day, the expression of VEGF decreased slightly, but still higher than the control group. The expression of VEGF was mainly found in the cytoplasm of vascular endothelial cells, osteoblasts, fibroblasts, and osteoclasts. CONCLUSIONS： Expression of VEGF may play an important role in the early remodeling of periodontal tissues during orthodontic tooth movement.