摘要
Objective：To investigate the effects of Compound Zhebei Granule（复方浙贝颗粒,CZG）combined with doxorubicin hydrochloride（adriamycin,ADM）on specific surface antigens in mice with KG-1a transplanted cells.Methods：A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude mice.Twenty-four tumor bearing mice were divided into 4groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each group.Drug administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 m L/10 g every other day.ADM group mice were intraperitoneally injected with ADM 1 mg/kg[conversion of adults,40 mg/（m^2·d）]every other day.High-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM（1 mg/kg）every other day.The administration period lasted for10 days.The tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋,in KG-1a cell line tumor xenografts were detected by flow cytometry.Results：Compared with the control and ADM groups,expression of CD34~＋CD38^-in the two CZG groups was significantly lower（P〈0.05）.Compared with the control group,expression of CD34~＋CD38^-CD123~＋in the two CZG groups decreased（P〈0.05）.The high-dose CZG group showed more obvious outcomes compared with the ADM group（P〈0.05）.Compared with the control and ADM groups,the expression of CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in the two CZG groups decreased（P〈0.05）.Conclusions：CZG combined with doxorubicin could reduce the expression of CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in KG-1a cell line tumor xenografts,which shows that CZG could target leukemia stem cells and play a role in chemosensitization.
Objective：To investigate the effects of Compound Zhebei Granule（复方浙贝颗粒,CZG）combined with doxorubicin hydrochloride（adriamycin,ADM）on specific surface antigens in mice with KG-1a transplanted cells.Methods：A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude mice.Twenty-four tumor bearing mice were divided into 4groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each group.Drug administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 m L/10 g every other day.ADM group mice were intraperitoneally injected with ADM 1 mg/kg[conversion of adults,40 mg/（m^2·d）]every other day.High-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM（1 mg/kg）every other day.The administration period lasted for10 days.The tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋,in KG-1a cell line tumor xenografts were detected by flow cytometry.Results：Compared with the control and ADM groups,expression of CD34~＋CD38^-in the two CZG groups was significantly lower（P〈0.05）.Compared with the control group,expression of CD34~＋CD38^-CD123~＋in the two CZG groups decreased（P〈0.05）.The high-dose CZG group showed more obvious outcomes compared with the ADM group（P〈0.05）.Compared with the control and ADM groups,the expression of CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in the two CZG groups decreased（P〈0.05）.Conclusions：CZG combined with doxorubicin could reduce the expression of CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in KG-1a cell line tumor xenografts,which shows that CZG could target leukemia stem cells and play a role in chemosensitization.
