大黄水提物与醇提物对实验性高脂血症小鼠血脂的影响研究

Study on effect of rhubarb water extract and ethanol extract on blood fat in experimental hyperlipidemia mice

目的探讨大黄水提物与醇提物单味应用与1∶1伍用对实验性高脂血症小鼠血清中血脂的影响。方法选取体质量20~25g昆明种雄性小鼠给予不同方法灌胃,分为对照组、模型组、实验1组(水提取)、实验2组(醇提取)、实验3组(水提取与醇提取1∶1配伍),实验28d后检测各组小鼠血清中三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平。结果实验1组小鼠TG、LDL-C水平低于模型组(P<0.05),HDL-C高于模型组(P<0.05);实验2组小鼠TC、TG、LDL-C水平低于模型组(P<0.05),HDL-C高于模型组(P<0.05);与实验1组比较,实验2组小鼠TG、LDL-C降低(P<0.05);与实验2组和实验1组比较,实验3组小鼠TC、TG、LDL-C均降低(P<0.05),HDL-C升高(P<0.05)。结论大黄用乙醇提取降血脂效果较好。

Objective To investigate the effect of single use and 1：1 combination use of rhubarb water extract and ethanol extract on blood fat in experimental hyperlipidemia mice. Methods Kunming male mice weighing 20--25 g were selected,given different methods of gavage and divided into the control group （Con）, model group （Mod）, test 1 group （Rhel, water extract）, test 2 group （Rhe2,ethanol extract） , test 3 group （water extract and ethanol extract 1：1 compatibility group）. The levels of serum total cholesterol （TC）,triglyceride （TG） ,low density lipoprotein cholesterol （LDL-C） and high-density lipoprotein cholesterol （HDL- C） in each group were detected on 28 d after experiment. Results The TG and LDL-C levels in the test 1 group were lower than those in the model group（P〈0.05） ,the HDL-C level was higher than that in the model group（P〈0.05） ;the levels of TC,TG and LDL-C in the test 2 group were lower than those in the model group,the HDL-C level was higher than that in the model group（P〉 0.05） ; compared with the test 1 group, the levels of TG and LDL-C in the test 2 group were decreased（P〈0.05） ;compared with the test 2 group and test 1 group,the levels of TC,TG and LDL-C in the test 3 group were decreased and the HDL-C level was increased（P〈0.05）. Conclusion Using ethanol for extracting rhubarb has better effect for reducing blood fat.