XP-endo Finisher清除椭圆形根管细菌生物膜的效果研究

Effectiveness of XP-endo Finisher on Biofilm Removal from Oval Root Canals

目的:比较XP-endo Finisher(XPF)和被动超声冲洗(passive ultrasonic irrigation,PUI),及两者联合应用清除椭圆形根管细菌生物膜的效果。方法:选取54颗成人离体单根管横截面为椭圆形的前磨牙,建立多菌种生物膜感染根管模型。厌氧培养4周后随机选取6颗为对照,扫描电镜(Scanning electron microscopy,SEM)确认多菌种生物膜形成。余牙应用Vortex Blue预备到40/.06后分为4个实验组(n=12),分别采用传统注射器冲洗(conventional needle irrigation,CNI)、PUI、XPF、PUI+XPF结合3%次氯酸钠溶液进行根管处理。采集样本根管尖、中、冠1/3SEM图像,计算残余细菌生物膜面积百分比。结果:细菌清除效率由高到低依次为PUI+XPF、XPF、PUI、CNI,前两者无统计学差异(P>0.05)。结论:XPF清除椭圆形根管生物膜的效果优于PUI和CNI,与PUI联合应用有加强效果但无显著差异。

Objective：To compare the effectiveness of XP-endo Finisher（XPF）in biofilm removal with passive ultrasonic irrigation（PUI）technique applied respectively or combinational using an infected oval-canal tooth model.Methods：Fifty-four extracted human premolars with single oval canals were selected.After growing multispecies biofilm for 4 weeks,6 teeth were selected randomly as controls and observed under scanning electron microscopy（SEM）to confirm the biofilm growth.The other teeth were instrumented using Vortex Blue files to size 40/.06 and then assigned to 4 groups（n=12）according to the irrigation protocol：CNI,PUI,XPF,and PUI＋XPF.3%NaOCl was used as the irritant.SEM images were taken from the apical,middle,and coronal third of the canal surface to evaluate the amount of residual biofilm.Results：The ranking of the effectiveness of biofilm removal from the most to the least was：PUI＋XPF,XPF,PUI and CNI with no statistical difference between PUI＋XPF and XPF（P0.05）.Conclusion：XPF was more effective in removing biofilm from oval root canals than PUI and CNI.The combination with PUI could slightly enhance the efficacy with no significant difference.