摘要
运用ISSR分子标记技术对古田县境内主要柿子种质进行遗传多样性分析,探究其亲缘关系。结果表明:14条UBC引物对15份材料共扩增出113个DNA位点,多态位点共93个,多态性带占比为82.30%。采用Ntsys-pc 2.10e软件分析计算,15份材料的相似系数为0.8230~0.5841。表明福建省古田县柿子种质资源的多样性较丰富。研究结果可为柿属种质资源的收集、鉴定和栽培利用提供参考。
The genetic diversity and genetic relationship of 15 persimmon materials in Gutian County were analyzed by ISSR molecu- lar marker technique. The results show that：total of 113 DNA loci,93 polymorphic loci and polymorphic band of 82. 30% of 15 persimmon materials were amplified by 14 UBC primers. The similarity coefficient of 15 points are 0. 8230 -0. 5841 that analyzed and calculated by Ntsys -pc 2. 10e software, which indicates that the diversity of persimmon germplasm resources is rich in Gutian County,Fujian Province. And which provided the reference to the collection, identification and cultivation of persimmon germplasm resources.
出处
《福建林业科技》
2018年第1期1-4,29,共5页
Journal of Fujian Forestry Science and Technology
基金
福建农林大学科技发展基金(农林大科[2015]36号)
关键词
柿
ISSR
遗传多样性
亲缘关系
Diospyros kaki L.
ISSR
genetic diversity
genetic relationship
