复方柴归方抗抑郁作用及其调控5-羟色胺代谢途径机制研究

Antidepressant effect of Compound Chaigui Prescription and its mechanism of regulating 5-HT metabolic pathway

目的研究复方柴归方抗抑郁作用及其调控5-羟色胺(5-HT)代谢途径的机制。方法 SD大鼠随机分为7组:对照组,模型组,化学药阳性对照组(文拉法辛0.035 g/kg),中药阳性对照组(舒肝解郁胶囊0.15 g/kg),复方柴归方高、中、低剂量(生药16.6、8.3、4.2 g/kg)组,复制慢性温和不可预知应激(CUMS)抑郁模型,造模同时ig给药4周,观察给药后大鼠体质量、糖水偏爱率、旷场行为;采用LC-MS方法检测血清中色氨酸(Trp)、5-HT、5-羟吲哚乙酸(5-HIAA)的量。结果复方柴归方可显著回调CUMS模型大鼠异常水平的体质量、糖水偏爱率、旷场行为和血清中Trp、5-HT、5-HIAA的量。结论复方柴归方抗抑郁作用明显,与文拉法辛或舒肝解郁胶囊作用相当,其作用机制可能与调控5-HT代谢途径有关。

Objective To study the antidepressant effect of Compound Chaigui Prescription and its mechanism of regulating 5-hydroxytryptamine（5-HT） metabolic pathway. Methods SD rats were randomly divided into seven groups： blank control group, model group, western medicine positive control group（venlafaxine 0.035 g/kg）, traditional Chinese medicine positive control group（Shugan Jieyu Capusle 0.15 g/kg）, high-, mid-, and low-doses of Compound Chaigui Prescription（crude drug 16.6, 8.3, and 4.2 g/kg）. And the rats were treated with chronic unpredictable mild stress（CUMS） for 4 weeks, the body weight, sugar preference rate and opening behavior were observed after administration; The levels of tryptophan（Trp）, 5-HT, and 5-hydroxyindole acetic acid（5-HIAA） in serum were measured by LC-MS. Results Compound Chaigui Prescription can significantly callback CUMS model rats abnormal level of body weight, sugar preference rate and opening behavior and the levels of Trp, 5-HT, and 5-HIAA in serum. Conclusion The Compound Chaigui Prescription has significant antidepressant effect, which is equivalent to the antidepressant effect of venlafaxine and Shugan Jieyu Capsule, and the mechanism may be related to the regulation of 5-HT metabolic pathway.