低分子壳寡糖对LPS诱导的小鼠颅骨吸收的抑制作用

Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides

目的 :研究低分子量壳寡糖(low-molecular chitosan oligosaccharide,LMCOS)对脂多糖(lipopolysaccharides,LPS)诱导的小鼠颅骨吸收模型的影响,评估其在小鼠颅骨吸收过程中的抑制作用。方法:将6周龄C57/BL6雄性小鼠随机分为5组,即磷酸缓冲液(PBS)组、LPS组、LPS+0.005%LMCOS组、LPS+0.05%LMCOS组和LPS+0.5%LMCOS组;每组5只,LPS的刺激量为10 mg/kg。小鼠浅麻醉后,于颅盖骨皮下注射相应试剂,每只注射300μL。每周小鼠头部皮下组织和颅骨骨膜之间注射试剂3次,2周后处死,取出颅盖骨进行Micro-CT扫描、H-E染色、TRAP染色。采用SPSS21.0软件包对数据进行单因素方差分析,观察骨破坏面积、破骨细胞的形成等骨缺损表现。结果:Micro-CT扫描、H-E染色、TRAP染色发现,与PBS组相比,LPS组小鼠颅骨表面的骨破坏十分明显;加入不同浓度的LMCOS后,颅骨骨破坏面积及破骨细胞数目随LMCOS浓度的升高而不断减少。结论:LMCOS具有抑制LPS诱导的小鼠颅骨骨吸收的作用,提示LMCOS对骨破坏性疾病可能具有一定的治疗作用。

PURPOSE： To study the effect of low molecular weight chitosan（low-molecular chitosan oligosaccharide,LMCOS） on lipopolysaccharides（LPS）-induced bone resorption in mice. METHODS： Six-week c57/bl6 male mice were randomly divided into 5 groups： phosphate buffer saline（PBS） group, LPS group, LPS＋0.005% LMCOS group, LPS＋0.05%LMCOS group and LPS ＋0.5% LMCOS group. There were 5 mice in each group, LPS was used at 10 mg/kg. After mild anesthesia, 300 μL corresponding reagents were injected into the skull bone. The mice were injected 3 times a week and sacrificed 2 weeks later. The skull bone was removed for micro-CT scan, H-E staining, TRAP staining. SPSS21.0 software package was used for statistical analysis. Bone damage area, osteoclast formation was observed and recorded. RESULTS：After LMCOS was added to LPS-induced skull resorption, bone destruction area decreased obviously; and with the increase of LMCOS concentration, bone destruction area and number of osteoclasts decreased gradually. CONCLUSIONS： LMCOS can inhibit the damage of skull bone in LPS-induced mice, which may have a therapeutic effect on osteolytic diseases.