体外细胞实验中药干预方法研究进展

Research progress of the traditional Chinese medicine intervention technique of the in vitro cell experiment

中药及复方的药理研究为中医中药的科学性提供证据,体外细胞培养技术在中药及复方的体外细胞实验中应用广泛,中药的复杂性使药物的添加方式成为研究的关键影响因素之一。目前应用的干预方法主要有3种:中药提取物/纯化物直接添加、中药血清药理学、中药血浆药理学。3种添加方法各有利弊,是否选择了合适的干预方法直接影响实验结果的可信度。国内研究多采取中药血清/血浆药理学,但是局限于口服中药复方的研究,同时其他给药途径的研究方法基础薄弱,需要归纳与总结。总之,中药干预方法的选择存在争议,需要进一步讨论。就此文章查阅近十几年来国内外文献,对体外细胞实验中中药的干预方法及选择进行总结,为今后相关实验研究提供参考。

Pharmacological study of compound Chinese medicine provides evidence for the science of Chinese medicine, As for medical herb research, in vitro cell culture technology is widely used. The complexity of traditional Chinese medicine makes the adding method become one of the key factors to the experiment. At present, there are three kinds of intervention methods： adding Chinese herbal extracts, serum pharmacology, plasma pharmacology. These methods have their own advantages and disadvantages, and the choice of appropriate intervention methods will directly affect the reliability of the experimental results. Domestic researches mostly adopt serum/plasma pharmacology, but it is limited to the study of oral Chinese herbal compound, and how to study traditional Chinese medicine useing other ways to absorb is not clear. In conclusion, the choice of Chinese medicine intervention methods is controversial and needs further discussion. By reviewing the domestic and foreign literatures in past ten years, this review summaries the intervention methods of traditional Chinese medicine in vitro cell experiment, and will provide a reference for the relevant experimental research in the future.