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雄激素诱导泰国斗鱼雄性化研究 被引量:3

Masculinization of Thailand betta( Bettasplendens) with Androgen Treatment
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摘要 传统方法培育的泰国斗鱼(Bettasplendens)苗种利用率较低(雄性率低),严重影响了生产效益.因此,急需寻求一种提高泰国斗鱼雄性率的新方法.本文通过雄激素(17α-甲基睾酮)投喂与早期浸泡的方法,研究了雄激素对泰国斗鱼雄性率的影响.利用浸泡方法处理的最佳时间和剂量分别为20天和100ug/L,处理10日龄仔鱼20天,雄性率提高到86%;投喂激素处理20和30天的结果没有显著性差异,均达到80%.另外,通过组织学和基因表达的比较研究,结果表明雄激素诱导雄鱼与正常发育雄鱼在组织学与基因表达水平上没有显著性差异.本研究结果为开发新的泰国斗鱼雄性化技术提供了新思路和研究基础. The traditional seed method of Thailand betta (Bettasplendens) led to low male rate and seriously affected the production efficiency of Thailand betta. Therefore, it is necessary to find a new method to increase the male rate for Thailand betta. In this study, Thailand betta was treated with androgen (17α-methyl testosterone ) by ways of exposure and feeding in the early stage to provide an effective method to increase the male rate of Thailand Betta. The results showed that the optimal time span and dose for exposure approach are 20 days and 100ug/L, which increase the male rate to 86%. According to the feeding treatment, there is no significant difference between 20 and 30 days' treatment, the male rates of which both are up to 80%. In addition, the histology and molecular expression analysis indicated that there is no significant difference between normal and androgen induced groups. This study proves that androgen induced method is reliable and applicable for Thailand betta seed production.
作者 袁洁怡 贺超 洪广 黄志鹏 林星桦 陈华谱 李水生 李广丽 陈燕 YUAN Jie-yi;HE Chao;HONG Guang;HUANG Zhi-peng;LIN Xing-ye;CHEN Hua-pu;LI Shui-sheng;LI Guang-li;CHEN Yan(Marine Ecology and Aquaculture Environment Key Laboratory of Zhanjiang City, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang Guangdong 524088, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao Shandong 266071,China;School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;Key Laboratory of Tropic Sea Fishery Protection and Utilization of Hainan Province, Hainan Tropical Ocean University, Sanya Hainan 572022, China)
出处 《海南热带海洋学院学报》 2018年第2期13-19,共7页 Journal of Hainan Tropical Ocean University
基金 国家自然科学基金项目(41706174) 广东省自然科学基金项目(2015A030313069 2016A030313743) "攀登计划"广东大学生科技创新培育专项资金项目(pdjh2017b0241) 湛江市财政资金科技竞争性分配项目(2016A03017) 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题项目(2016LMFS-B12)
关键词 泰国斗鱼 雄性率 雄激素 组织学 分子表达 Thailand betta( Betta splendens ) male rate androgen histology molecular expression
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