普鲁兰多糖/槐糖脂抑菌保鲜膜的制备

Preparation of antimicrobial preservative film based on pullulan and sophorolipids

目的:为替代传统不可再生塑料保鲜膜,采用经济环保原料制作性能优良的抑菌保鲜膜,并确定其最佳配方。方法:在最佳成膜条件下,采用单因素实验分析普鲁兰多糖、槐糖脂和甘油浓度对成膜性能的影响,并考察了槐糖脂的抑菌浓度。在此基础上设计正交实验,确定抗菌保鲜膜原料的最佳配比,并验证膜液及保鲜膜的保鲜性能。结果:槐糖脂对金黄色葡萄球菌抑制效果显著(p<0.01),2.4 g/L时达到100%抑制;对大肠杆菌和白色假丝酵母菌菌有不同程度的抑制作用,4.0 g/L时抑菌率分别为28.75%和14.53%。确定了膜液的最佳配方为普鲁兰多糖18 g/L、槐糖脂3.6 g/L、甘油8.75 g/L。该膜液及所成的抗菌保鲜膜对樱桃的保鲜效果与对照组相比效果显著(p<0.01)。结论:将槐糖脂生物表面活性剂作为可抗菌保鲜膜的原料,为绿色抗菌保鲜膜的制备工艺提供新的理论依据。

Objective：To replace the traditional non-renewable plastic wrap, the performance of antibacterial plastic film was studied using the economical and environment- friendly raw materials. Methods ： Under the best film- forming conditions, the effects of the concentrations of pullulan polysaccharides,sophorolipids and glycerin on the properties of preservative film were analyzed by single factor experiments. Meantime, the optimal inhibitory concentration of sophorolipids was investigated.Then, their suitable concentrations were determined by orthogonal experiment.After that,the fresh-keeping property of preservative solution and film were investigated.Results ：The antimicrobial activity of sophorolipids on Staphylococcus aureus was significant （p 〈 0.01 ） .It was 100% inhibited when the concentration of sophorolipids was 2.4 g/L.The sophorolipids have different inhibition on Escherichia coli and Candida albicans.When the concentration of sophorolipids was 4.0 g/L, the inhibition rate against E.coli and C.albicans were 28.75% and 14.53% ,respectively.The best formula of preservative solution was determined as 18 g/L of pullulan polysaccharides,3.6 g/L of sophorolipids and 8.75 g/L of glycerin.Both the preservative solution and the preservative fihn have good effects on fresh keeping of cherries （p 〈 0.01 ）.Conclusion：Using bio-suffactant sophorolipids as raw materials of antimicrobial preservative film could provide a new theoretical basis for the preparation process of green antimierobial cling fihn.