规模化驴场几种重要传染病的调查研究

Investigatio of Several Important Pathogens of Infectious Diseases in Scaled Donkey Farms

为了解马流感(EI)、马鼻肺炎(ER)、布鲁菌病(Brucellosis)、日本脑炎(JE)和沙门菌病等重要传染病在养驴场的危害,在山东聊城市13个规模驴场采集血液、棉拭子和病料等样品共226份,并分别使用双重RT-PCR、real-time PCR、胶体金、C-ELISA、鉴别培养基等方法,对这些传染病进行了调查。结果表明,H3N8亚型EIV为阴性,马疱疹病毒4型(EHV-4)感染率为3.6%(2/55)、马疱疹病毒1型(EHV-1)为阴性,血清样品中的布鲁菌和JEV抗体检测结果为阴性,沙门菌属检测显示阴性。验证了这些检测方法对驴群样品检测的适用性,结果表明这些规模化驴场饲养的驴处于良好的健康状态。

226 samples of blood, cotton swabs and diseased materials were collected from 13 scaled donkey farms in Liaocheng, Shandong for detecting the pathogens of equine influenza （EI）,Equine rhinopneumo- nia（ER）, Brucellosis,Japanese B encephalitis （JBE） and Salmonellosis. The methods of double RT-PCR, real time-PCR, colloidal gold test, C-ELISA and differential medium were used to detect these pathogens of infectious diseases. The results showed that EIV H3N8 subtype was negative, EHV-4 infection rate was 3.6% （2/55）, EHV-1 was negative, Brucella and JBEV antigen in serum samples were negative, Salmonella detection was negative.This suggests that these commonly used methods are appropriate for detecting donkey samples and these donkeys in scaled farms are in good health.