七氟醚和丙泊酚对人体精子运动功能和体外获能的影响

The effects of sevoflurane and propofol on human sperm motility and capacitation in vitro

目的 观察吸入麻醉药七氟醚和静脉麻醉药丙泊酚在临床浓度下对人体精子运动功能和体外获能的影响.方法 正常成年男性精液标本经Percoll密度梯度法离心优选后沉淀,沉淀精子置于人体精子体外获能培养基,调节精子密度为（30 ～50）×10^6/ml,随机分为对照组（C组）、七氟醚组（S组）和丙泊酚组（P组）,七氟醚组根据暴露环境中七氟烷浓度不同,分为低浓度组（S1组,1.8％）和高浓度组（S2,2.2％）;丙泊酚组根据培养基中丙泊酚浓度不同,分为低浓度组（P1组,1.8 μg/ml）和高浓度组（P2组,2.4μg/ml）.在37℃孵箱中培养5h,采用计算机辅助精子分析系统（CASA）评价精子运动参数,包括：精子运动活力（a＋b级）％、曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）和头部侧向运动平均振幅（ALH）;采用金霉素荧光染色技术评价精子体外获能,以（B＋AR）％表示已发生获能的精子比例.结果 1.8％、2.2％七氟醚和1.8、2.4μg/ml丙泊酚分别作用于精子5h以后,精子的运动活力（a＋b级）％以及VCL、VSL、VAP、ALH都有明显下降,且对精子运动功能的影响呈现剂量依赖关系（P=0.017）;相同临床效应浓度,七氟醚对精子运动功能的抑制作用明显强于丙泊酚（P=0.026）;相同临床效应浓度下,七氟醚对精子获能的抑制作用显著强于丙泊酚组（P=0.011）.结论 七氟烷和丙泊酚临床相关浓度暴露,对人体精子运动功能和体外获能均具有抑制作用,随着暴露浓度的增加,抑制作用增强;七氟烷对精子运动功能和体外获能的抑制作用强于丙泊酚.

Objective To investigate the effects of sevoflurane and propofol on human sperm motility and capacitation at the clinic concentration in vitro.Methods Sperm samples were obtained from normal adults and prepared with discontinuous percoll gradient centrifugation technique,adjusting the sperm density at （30-50） × 10^6/ml.The sperm samples were randomly divided into control group （C group）,sevoflurane group （S group） and propofol group （P group）,according to the concentration of sevoflurane,S group was divided into low concentration group （S1 group,1.8% sevoflurane） and high concentration group （S2 group,2.2% sevoflurane）,according to the concentration of propofol,P group was divided into low concentration group （P1 group,1.8 μg/ml） and high concentration group （P2 group,2.4 μg/ml）,all the samples were incubated for 5 h in an airtight glass container filled with air at 37 ℃.Then human sperm motility waft examined in vitro at 37 ℃ and analyzed by the computer-assisted sperm analysis （CASA）,including sperm motility （a ＋ b） %,curvilinear velocity （VCL）,straight line velocity （VSL）,average path velocity （VAP） and amplitude of lateral head displacement （ALH）.The capacitation effect was assessed by using the chlortetracycline （CTC） staining,（B ＋ AR） % indicates the proportion of sperm capacitation has occurred.Results Compared with control group,after incubation of the sperm samples with 2% and 4% sevoflurane,4 μg/ml and 8 μg/ml propofol for 5 h,the motility of sperm （a ＋b）% and VCL,VSL,VAP,ALH has decreased,and the impact on sperm motility in a dose-dependent manner;both sevoflurane and propofol at clinical concentrations could inhibit sperm capacitation in a dose-dependent manner（P =0.017）;at the same clinical effect concentrations,inhibitory effects of sevoflurane on sperm motility was stronger than propofol （P =0.026）;about sperm capacitation,at the same clinical effect concentrations,inhibitory effects of sevoflurane on sperm capacitation was significantly stronger than the propofol （P =0.011）.Conclusion Sevoflurane and propofol at clinically relevant concentrations have significant inhibitory effects on human sperm motility and capacitation in a dose-dependent manner.Sevoflurane has stronger inhibitory effect than propofol.